Acetylsalicylic Acid

Acetylsalicylic Acid

CAS Number50-78-2
Molecular FormulaC9H8O4
Molecular Weight180.159
InChI KeyBSYNRYMUTXBXSQ-UHFFFAOYSA-N
LogP1.2
Synonyms
  • aspirin
  • ACETYLSALICYLIC ACID
  • 2-Acetoxybenzoic acid
  • 50-78-2
  • 2-(Acetyloxy)benzoic acid
  • Acetylsalicylate
  • O-Acetylsalicylic acid
  • o-Acetoxybenzoic acid
  • Acetosal
  • Acylpyrin
  • Polopiryna
  • Ecotrin
  • Salicylic acid acetate
  • Acenterine
  • Acetophen
  • Colfarit
  • Easprin
  • o-Carboxyphenyl acetate
  • Enterosarein
  • Aceticyl
  • Acetonyl
  • Acetosalin
  • Acetylin
  • Aspergum
  • Aspirdrops
  • Benaspir
  • Micristin
  • Pharmacin
  • Premaspin
  • Salcetogen
  • Temperal
  • Ecolen
  • Empirin
  • Endydol
  • Rhodine
  • Saletin
  • Rheumintabletten
  • Solprin acid
  • Acidum acetylsalicylicum
  • Acetisal

Applications:

USP Methods for the Analysis of Aspirin (Acetylsalicylic acid (ASA)) Using Legacy L1 Column

June 21, 2012

 

Application Notes: Aspirin is one of the oldest analgesics. While it is one of the oldests analgesics, it is still widely used today, and is still one of the most common drugs. According to the USP methods, aspirin contains not less than 99.5% and no more than 100.5 percent of aspirin calculate on a dried basis. The USP HPLC method for the separation of aspirin was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP.

Application Columns: Legacy L1 C18 HPLC column

Application compounds: Aspirin and salicylic acid

Mobile phase: 20 mM AmFm pH 3.0/MeCN- 75/25

Detection technique: UV

Reference: USP35: NF30

Condition

Column Legacy L1, 4.6×300 mm, 5 µm, 100A
Mobile Phase MeCN – 25%
Buffer AmFm pH 3.0 20 mM –  75%
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophobic, Ionizable
Analyzing Compounds Aspirin (Acetylsalicylic acid (ASA))

 

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Select options
Application Analytes:
Acetylsalicylic Acid
Aspirin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Components of Excedrin (Benzoic acid, Acetaminophen, Caffeine, Aspirin)

July 16, 2009

Excedrin is over-the-counter pain reliever containing acetaminophen, caffeine and aspirin as active ingredients of this drug composition. Acetaminophen (paracetamol) is used as analgesic and pain reliever. It is a neutral compound with low hydrophobicity. Aspirin or acetylsalicylic acid is used as analgesic and anti-inflammatory component of many OTC compositions. It is weakly acidic and slightly hydrophobic compound. Caffeine is xanthine alkaloid which is psychoactive stimulant drug. All four compounds are separated on mixed-mode Primesep 100 HPLC column with acetonitrile/water/TFA mobile phase. In this HPLC application compounds are retained by reversed phase mechanism. This HPLC method is short and robust.

Condition

Column Primesep 100, 3.2×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 0.5 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Acid, Hydrophilic, Ionizable
Analyzing Compounds Benzoic acid, Acetaminophen, Caffeine, Aspirin

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Acetylsalicylic Acid
Aspirin
Benzoic Acid
Caffeine

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.