Adenosine

Adenosine

CAS Number58-61-7
Molecular FormulaC10H13N5O4
Molecular Weight267.245
InChI KeyOIRDTQYFTABQOQ-KQYNXXCUSA-N
LogP-1.05
Synonyms
  • Adenosine
  • 58-61-7
  • (-)-Adenosine
  • 9H-Purin-6-amine, 9-β-D-ribofuranosyl-
  • 9-β-D-Ribofuranosyl-9H-purin-6-amine
  • 9-β-D-Ribofuranosyladenine
  • Adenine riboside
  • Adenocard
  • Adenocor
  • Adenoscan
  • Adenosin
  • adenosina
  • Adrekar
  • Boniton
  • D-Adenosine
  • NSC 7652
  • Nucleocardyl
  • Riboadenosine
  • Sandesin
  • β-Adenosine
  • β-D-Adenosine
  • β-D-Ribofuranose, 1-(6-amino-9H-purin-9-yl)-1-deoxy-
  • β-D-Ribofuranoside, adenine-9
  • Caswell No. 010B
  • EINECS 200-389-9
  • 9H-Purin-6-amine, 9beta-D-ribofuranosyl-
  • beta-D-Ribofuranose, 1-(6-amino-9H-purin-9-yl)-1-deoxy-
  • beta-D-Ribofuranoside, adenine-9
  • NSC 627048
  • UNII-K72T3FS567
  • (2R,3R,4R,5R)-2-(6-aminopurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol
  • (2R,3R,4R,5R)-2-(6-aminopurin-9-yl)-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
  • (2R,3R,4R,5R)-2-(6-aminopurin-9-yl)-5-methylol-tetrahydrofuran-3,4-diol
  • (2R,3R,4S,5R)-2-(6-amino-9H-purin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol
  • (2R,3R,4S,5R)-2-(6-aminopurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol
  • 1-(6-Amino-9H-purin-9-yl)-1-deoxy-beta-D-Ribofuranose
  • 1-(6-Amino-9H-purin-9-yl)-1-deoxy-beta-delta-Ribofuranose
  • 6-Amino-9-beta-D-ribofuranosyl-9H-purine
  • 6-Amino-9beta-D-ribofuranosyl-9H-purine
  • 6-Amino-9beta-delta-ribofuranosyl-9H-purine
  • 9-beta-D-Arabinofuranosyladenine
  • 9-beta-D-Ribofuranosidoadenine
  • 9-beta-D-Ribofuranosyl-9H-purin-6-amine
  • 9-beta-D-Ribofuranosyladenine
  • 9-beta-delta-Arabinofuranosyladenine
  • 9-beta-delta-Ribofuranosidoadenine
  • 9-beta-delta-Ribofuranosyl-9H-purin-6-amine
  • 9-beta-delta-Ribofuranosyladenine
  • 9beta-D-Ribofuranosyladenine
  • 9beta-D-ribofuranosyl-9H-Purin-6-amine
  • 9beta-delta-Ribofuranosyladenine
  • 9beta-delta-ribofuranosyl-9H-Purin-6-amine
  • Ade-Rib
  • Adenine Deoxyribonucleoside
  • Adenine nucleoside
  • Adenine-9beta-D-Ribofuranoside
  • Adenine-9beta-delta-Ribofuranoside
  • Adenyldeoxyriboside
  • Ado
  • Deoxyadenosine
  • Desoxyadenosine
  • Myocol
  • b-D-Adenosine
  • beta-Adenosine
  • beta-D-Adenosine
  • beta-delta-Adenosine
  • 46946-45-6
  • 46969-16-8

Applications:

HPLC Method for Separation of Adenine, Deoxyadenosine and Adenosine on BIST B+ Column

November 28, 2022

HPLC Method for Separation of Adenine, Deoxyadenosine and Adenosine on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Separation of Adenine, Deoxyadenosine and Adenosine on BIST B+ Column
HPLC Method for Separation of Adenine, Deoxyadenosine and Adenosine on BIST B+ by SIELC Technologies.

Condition

ColumnBIST B+, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN – 85%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 260 nm
Peak Retention Time2.8, 3.2, 4.3 min

Description

Class of CompoundsNucleosides
Analyzing CompoundsAdenine, Deoxyadenosine and Adenosine

Application Column

BIST B+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Adenine
Adenosine
Deoxyadenosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Adenosine and Deoxyadenosine on Newcrom AH Column

May 26, 2021

Separation type: Liquid Chromatography Mixed-mode


HPLC.cloud
View on hplc.cloud

HPLC-Separation-of-Adenosine-Deoxyadenosine-on-Newcrom-AH-Column

High Performance Liquid Chromatography (HPLC) Method of Adenosine and Deoxyadenosine.

Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: adenosine and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and ammonium formate (AmFm) buffer. Detection can be achieved with UV 260 nm, mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (,.CAD).

Condition

Column Newcrom AH, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer  AmFm pH 3.0 – 10 mM
Flow Rate 1.0 ml/min
Detection UV, 260 nm

 

Description

Class of Compounds
Nucleatide
Analyzing Compounds Adenosine,  Deoxyadenosine

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Adenosine
Deoxyadenosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine on Newcrom AH Column

May 25, 2021

Separation type: Liquid Chromatography Mixed-mode


HPLC.cloud
View on hplc.cloud

Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: guanosine, deoxyguanosine, adenosine, and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and H3PO4 as a buffer. UV detection at 210 nm.

Condition

Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Nucleoside,  Hydrophilic, Ionizable
Analyzing Compounds Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Adenosine
Deoxyadenosine
Deoxyguanosine
Guanosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Adenosine, Cordycepin and Adenine on Newcrom AH Column

April 8, 2020


HPLC.cloud
View on hplc.cloud

 

Due to cordycepin having a very similar structure to adenosine, it has shown to have inhibitive properties on the COVID-19 coronavirus. However, due to their similar structures, the separation of the two sugars can be challenging. Both sugars can be separated isocratically in about six minutes on the Newcrom AH mixed-mode column, which has both hydrophobic and cationic exchange properties. The mobile phase consists of acetonitrile (ACN, MeCN) and water with ammonium formate as a buffer which makes it mass-spec (MS) compatible. It can also be UV detected at 260nm.

 

Condition

Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV 260 nm,  MS-compatible mobile phase

Description

Class of Compounds Hydrophilic, Drug, Xanthine, Nucleobase
Analyzing Compounds Adenosine,  Cordycepin, Adenine

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Adenine
Adenosine
Cordycepin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Model Compounds in Reversed-Phase and Mixed-Mode

April 25, 2019

Separation type: Liquid Chromatography Mixed-mode








 
Many compounds are difficult, if not impossible, to separate on reverse-phase columns in HPLC. Other compounds cannot be separated on ion-exchange columns. That’s where the mixed-mode columns come in. By using a stationary phase with both hydrophobic and ion-exchange properties, allows the chromatographer to have additional controls over separation conditions. Here, we demonstrate the separation of compounds that can’t be achieved on a C18 column. By using both an organic gradient and buffer gradient of ammonium formate (AmFm), we can separate structurally similar compounds that can’t be separated on a reverse-phase column alone.




 

Condition

Column Primesep 100, 3,2×50 mm, 2,7 µm, 100A
Mobile Phase Gradient  MeCN – 10-60%, 5 min
Buffer Gradient AmFm pH 3.5- 30 – 70 mM, 5 min
Flow Rate 1.2 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug,  Basic, Hydrophilic, Hydrophobic, Ionizable.
Analyzing Compounds Adenosine, 3,4-Difluroaniline, 4-Amino-2-chloropyridine, 5-Aminoindole, 4-Amino-3-chloropyridine, 2-Amino 5-methylthiadiazole, 4-Ethylaniline

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2-Amino-5-Methylthiadiazole
2-Amino-5-methyl-thiazole
3,4-Difluoroaniline
4-Amino-2-Chloropyridine
4-Amino-3-Chloropyridine
4-Ethylaniline
5-Aminoindole
Adenosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Nucleosides and Deoxynucleosides

July 23, 2012

Condition

Column Sharc 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer AmFm, Formic acid
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Thymidine, Uridine, Deoxyadenosine, Adenosine, Deoxyguanosine, Guanosine, Deoxycytidine, Cytidine

 

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
Adenosine
Cytidine
Deoxyadenosine
Deoxycytidine
Deoxyguanosine
Guanosine
Thymidine
Uridine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Adenosine and Adenine Using the Hydrogen Bonding Method

June 18, 2012

 

 

Application Notes: Nucleosides are glycosylamines consisting of a nucleobase linked to a ribose or a deoxyribose sugar. Nucleoside are building blocks for DNA and RNA. These compounds are very polar in nature and contain groups available for hydrogen bonding interactions. Method for separation of adenine and adenosine were developed using a hydrogen-bonding method. There is a strong correlation between retention time for adenine/adenosine and the mobile phase composition, which consists of acetonitrile and methanol. Order of elution for compounds depends on the amount of acetonitrile and methanol.  Furthermore, ellution of adenine and adenosine can be reversed based on the composition of the mobile phase. Our method is compatible with LC/MS and preparative chromatography.

Condition

Column Sharc 1, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer AmFm, Formic acid
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Adenosine, Adenine

 

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
Adenine
Adenosine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Thymidine, Uridine, Adenosine, Guanosine, and Cytidine Using the Hydrogen Bonding Method

June 15, 2012

 

Application Notes: Nucleosides are glycosylamines consisting of nucleobase linked to ribose or deoxyribose sugar and are building blocks for DNA and RNA. These compounds are very polar and contain groups available for hydrogen bonding interaction. Thymidine, uridine, adenosine, guanosine and cytidine were separated using a hydrogen-bonding method. There is a strong correlation between the retention time and mobile phase composition. The strength of hydrogen-bonding interaction increases as the number of hydroxyls in the analytes increase. Additionally the order of elution for compounds depends on the ratio of the mobile phases: acetonitrile and methanol. Our method is compatible with LC/MS and preparative chromatography.

Application Columns: SHARC 1, 3.2×100 mm, 5 um, 100A, To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site.

Application Compounds: Thymidine, uridine, adenosine, guanosine and cytidine

Condition

Column Sharc 1, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer AmFm, Formic acid
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Thymidine, Uridine, Adenosine, Guanosine, Cytidine

 

 

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
Adenosine
Cytidine
Guanosine
Thymidine
Uridine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Nucleic Bases at pH 4 and 5 on Obelisc N

March 3, 2007


Nucleic bases are biological compounds found in genetic molecules (DNA, RNA). They can be separated on an Obelisc N column, which offers very polar characteristics and can be used with positively or negatively charged groups. Closely-eluted adenosine and uridine can be further separated by simply adjusting the pH of the mobile phase. Mobile phase is water and acetonitrile (MeCN, ACN) with Ammonium Acetate as buffer. UV detection at 250nm.

Condition

Column Obelisc N, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN -90%
Buffer AmAc
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Uracil, Uridine, Adenosine, Guanosine, Cytidine, Cytosine

 

 

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Adenosine
Cytidine
Cytosine
Guanosine
Uracil
Uridine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.