Compounds  →  alpha-Aminobutyric Acid

Separation of alpha-Aminobutyric Acid

HPLC Separation of Isomers of Aminobutyric Acids



Primesep C separates the isomers of aminobutyric acids by a combination of reversed-phase and ionic interaction mechanisms. alpha-Aminobutyric acid, beta-Aminobutryic acid, and gamma-Aminobutyric acid (GABA) are baseline resolved without ion-pair reagents. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) ammonium acetate with evaporative light scattering detection (ELSD).



Application Analytes:

Aminobutyric Acids
beta-Aminobutyric Acid
alpha-Aminobutyric Acid
gamma-Aminobutyric Acid (GABA)

Application Detection:

ELSD/MS Detection

HPLC Separation of alpha-Aminobutyric, beta-Aminobutyric, and gamma-Aminobutyric acids on Obelisc N


GABA (neurotransmitter) and its isomers are polar zwitter-ionic compounds. Due to the position of amino-groups, all three compounds show different polar and basic properties. The isomers of aminobuturic acid are separated on an Obelisc N HILIC/cation-exchange column. Buffer concentration has a different effect on retention of alpha-, beta-, and gamma-aminobutyric acid. This general and robust method can be used for separation of other polar and ionizable compounds and isomers by mixed-mode chromatography.



Application Analytes:

Zwitterion
beta-Aminobutyric Acid
alpha-Aminobutyric Acid
gamma-Aminobutyric Acid (GABA)

Application Detection:

ELSD/MS Detection

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