Aspartame

Aspartame

CAS Number22839-47-0
Molecular FormulaC14H18N2O5
Molecular Weight294.307
InChI KeyIAOZJIPTCAWIRG-QWRGUYRKSA-N
LogP-2.7
Synonyms
  • Aspartame
  • Nutrasweet
  • Asp-phe-ome
  • Asp-Phe methyl ester
  • Aspartam
  • Aspartamum
  • Aspartamo
  • L-Aspartyl-L-phenylalanine methyl ester
  • Aspartylphenylalanine methyl ester
  • Dipeptide sweetener
  • Canderel
  • Methyl aspartylphenylalanate
  • Sweet dipeptide
  • 1-Methyl N-L-alpha-aspartyl-L-phenylalanate
  • Aspartam [INN-French]
  • Aspartame, L,L-alpha-
  • Aspartamum [INN-Latin]
  • Aspartamo [INN-Spanish]
  • N-L-alpha-Aspartyl-L-phenylalanine 1-methyl ester
  • UNII-Z0H242BBR1
  • 3-Amino-N-(alpha-methoxycarbonylphenethyl) succinamic acid
  • H-Asp-Phe-OMe
  • CCRIS 5456
  • CHEBI:2877
  • 3-Amino-N-(alpha-carboxyphenethyl)succinamic acid N-methyl ester
  • SC 18862
  • HSDB 3915
  • Methyl L-aspartyl-L-phenylalanine
  • EINECS 245-261-3

Applications:

HPLC Separation of Amino Acids on Obelisc R Column

October 4, 2007


Closely related compounds like amino acids can be separated on an Obelisc R column by various buffers depending on the amount of baseline separation required. By choosing different buffers, the separation between compounds can be adjusted based on application needs, especially those that require low organic concentration in the mobile phase. UV detection at 250nm.

Condition

Column Obelisc R, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Amino acids

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
3-Aminobenzoic Acid
Aspartame
Phenylalanine
Tryptophan

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Bufferless Ion Separation (BLIS™) Chromatography of Aspartame: Organic Modifier Effect

January 30, 2005

An organic modifier effect of changing acetonitrile percentage the mobile phase is demonstrated on a Primesep C HPLC column in a bufferless ion (BLIS™) separation. With decreasing percent acetonitrile, the artificial sweetener, Aspartame, first deceases in retention time and then increases. The changing mobile phase composition changes the retention mechanism from hydrophobic and BLIS™interactions, to BLIS™ alone , to BLIS™ and HILIC (hydrophilic liquid chromatography) interactions. Aspartame is composed of the two amino acids, aspartic acid and phenylalanine as the ethyl ester. Mobile phases composed of water and acetonitrile (MeCN, ACN,) and ultraviolet (UV) detection was used.

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Aspartame
Nutrasweet

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.