Compounds  →  Aspartic Acid

Separation of Aspartic Acid

Amino Acids Analysis in Acid Gradient Condition



11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).



Application Analytes:

Alanine
Amino Acids
Arginine
Aspartic Acid
Cysteine
Glutamic Acid
Histidine
Isoleucine
Lysine
Methionine
Phenylalanine
Valine

Application Detection:

ELSD/MS Detection

HPLC Separation of Polar Compounds





Application Analytes:

Aspartic Acid
Dopamine
Glutamic Acid
Norepinephrine
5-Hydroxytryptophan
gamma-Aminobutyric Acid (GABA)

Application Detection:

ELSD/MS Detection

Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)





Application Analytes:

Aspartic Acid
Alanine
Methionine
Valine
Leucine
Amino Acids

Application Detection:

UV Detection

Effect of both pH and Organic Content on a Separation of Sugars, Amino Acids, and Carboxylic Acids


In mixed-mode HILIC chromatography, selectivity of separation can be adjusted by amount of acetonitrile, amount of buffer and buffer pH. Buffer concentration and pH will affect retention of ionizable compounds to a different degree. Retention of neutral compounds can be adjusted by the amount of acetonitrile. Carboxylic acid, three amino acids and two sugars are separated by combination of HILIC and ion-exchange mechanisms. Compounds can be monitored by ELSD, Corona (CAD), LC/MS or low UV. UV-transparent mobile phase /buffer is required for UV monitoring of this mixed-mode separation. This HPLC method can be adopted as general approach for analysis of sugars, amino acids and carboxylic acids.



Application Analytes:

Aspartic Acid
Phenylalanine
Succinic Acid

Application Detection:

ELSD/MS Detection

HPLC Separation of Aspartic Acid and Asparagine on Obelisc R Column


Two amino acids, aspartic acid and asparagine, are separated on an Obelisc R column by combination of reverse-phase and ion-exchange mechanism. Method can be used for analysis of these and other underivatized amino acids by HPLC with UV, ELSD, CAD and LC/MS detection.



Application Analytes:

Asparagine
Aspartic Acid

Application Detection:

ELSD/MS Detection

HPLC Separation of Lysine and Arginine from Other Amino Acids

 

Application Notes: Amino acids are polar ionic compounds which are not retained on reversed-phase column without ion-pairing reagent. In our application, lysine and arginine can be separated from other amino acids. Amino acids with a pH between 3 and 5 and with one basic and one acidic group become very polar. Therefore these amino acids don’t have strong ion-exchange interaction with Primesep C stationary phase. Amino acids with two amino groups still carry positive net charge and can interact with stationary phase by cations-exchange mechanism. pH variation of the mobile phase can be an effective tool to adjust selectivity of separation for zwitter-ionic, basic and acidic compounds. This method can be used for separation of mono-charged compounds from compounds having an extra charge.

Application Columns: Primesep C
Application compounds: Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine
Detection technique: UV, LC/MS, ELSD/CAD



Application Analytes:

Alanine
Arginine
Asparagine
Aspartic Acid
Glutamic Acid
Glycine
Leucine
Lysine
Phenylalanine
Proline
Tyrosine

Application Detection:

UV Detection
ELSD/MS Detection

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