| CAS Number | 515-30-0 |
|---|---|
| Molecular Formula | C9H10O3 |
| Molecular Weight | 166.176 |
| InChI Key | NWCHELUCVWSRRS-UHFFFAOYSA-N |
| LogP | 1 |
| Synonyms |
Applications:
UV-Vis Absorption Spectrum of Atrolactic acid
December 18, 2025
If you are looking for optimized HPLC method to analyze Atrolactic Acid check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Separation of Mixture of Atropine, Atrolactic Acid and Pralidoxime Chloride
April 15, 2019
HPLC Method for Atropine, Atrolactic Acid, Pralidoxime Chloride on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Atropine, Atrolactic Acid, Pralidoxime Chloride.
Atropine is a tropane alkaloid with the chemical formula C17H23NO3. It is an anticholinergic medication that is used to treat nerve agent, pesticide poisoning, slow heartrate, and is also used to decrease saliva production. Typically, it is administered through injection.
Atrolactic Acid is a 2-hydroxy monocarboxylic acid with the chemical formula C9H10O3. It can cause irritation to eyes, skin, and the respiratory system.
Pralidoxime Chloride, also known as 2-PAM, is a cholinesterase reactivator with the chemical formula C7H9N2O+. It is used as an antidote for poisoning by organophosphate pesticides and nerve agents.
Atropine, Atrolactic Acid, Pralidoxime Chloride can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
| Column | Primesep 200, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Gradient H3PO4 – 0.1-0.3%, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Drug, Acid, Base, Hydrophilic, Hydrophobic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Atropine, Atrolactic Acid, Pralidoxime Chloride |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Atropine
Pralidoxime Chloride
HPLC Determination of Atrolactic Acid on Primesep 200 Column
April 5, 2019
HPLC Method for Atrolactic Acid on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Atrolactic Acid.
Atrolactic Acid is a 2-hydroxy monocarboxylic acid with the chemical formula C9H10O3. It can cause irritation to eyes, skin, and the respiratory system.
Atrolactic Acid can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
| Column | Primesep 200, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Drug, Acid, Hydrophobic |
| Analyzing Compounds | Atrolactic Acid |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
