Benzalkonium chloride

Benzalkonium chloride

CAS Number63449-41-2
Molecular FormulaC6H5CH2N(CH3)2RCl
Synonyms
  • benzethonium chloride
  • 121-54-0
  • Hyamine
  • Phemeride
  • Phemerol Chloride
  • Quatrachlor
  • Benzethoniumchloride
  • Hyamine 1622
  • Banagerm
  • Phemerol
  • Phemithyn
  • Disilyn
  • Kylacol
  • Diapp
  • Polymine D
  • Benzetonium chloride
  • Anti-germ 77
  • Benzethonii chloridum
  • Antiseptol
  • Cloruro de benzetonio
  • Inactisol
  • Neostelin green
  • Chlorure de benzethonium
  • Sanizol
  • Benzethonium chloride 1622
  • Microklenz
  • p-tert-Octylphenoxyethoxyethyldimethylbenzylammonium chloride
  • NSC-20200
  • 5929-09-9
  • benzyl-dimethyl-[2-[2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethoxy]ethyl]azanium;chloride
  • Diisobutylphenoxyethoxyethyl dimethyl benzyl ammonium chloride
  • Diisobutylphenoxyethoxyethyldimethyl benzyl ammonium chloride
  • CHEBI:31264
  • Phemerol chloride monohydrate

Applications:

HPLC MS Method for Analysis of Benzalkonium Chloride on Primesep SB  Column

February 2, 2024

HPLC Method for Analysis of Benzalkonium chloride on Primesep SB by SIELC Technologies

HPLC Method for Analysis of Benzalkonium chloride on Primesep B Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzalkonium chloride

Benzalkonium chloride is a quaternary ammonium compound and belongs to the class of chemicals known as cationic surfactants. It is often used as a disinfectant, antiseptic, and preservative in various products, including pharmaceuticals, personal care products, and surface disinfectants.

Function: It has antimicrobial properties and is effective against bacteria, viruses, and fungi. It disrupts the cell membranes of microorganisms, leading to their inactivation.

Common Uses:

  • Disinfection of surfaces in healthcare settings.
  • Preservative in ophthalmic solutions and nasal sprays.
  • Ingredient in some antiseptic wipes and hand sanitizers.
  • Preservation agent in various personal care products.

Variants: There are different variants of Benzalkonium chloride with varying alkyl chain lengths, and the specific composition may affect its properties and applications.

Benzalkonium can be retained, and analyzed using a Primesep SB mixed-mode stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using UV and LC MS positive mode

ColumnPrimesep SB, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 30 – 70%, 10 min
BufferAmmonium formate pH 3.0 – 40 mM
Flow Rate0.2ml/min
DetectionUV 262 nm, SIM + 276, 304, 332, 360
Samples0.0025 mg/mL in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*1 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Alkaloid, Quaternary ammonium
Analyzing CompoundsBenzalkonium chloride

Application Column

Primesep SB

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Benzalkonium chloride

Application Detection:
UV Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Hydrotopic, Cationic and Anion Surfactants on Newcrom BH Column

July 10, 2023

HPLC Method for Analysis of Hydrotopic, Cationic and Anion Surfactants on Newcrom BH by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Hydrotopic, Cationic and Anion Surfactants

Surfactants, also known as surface-active agents, are compounds that lower the surface tension (or interfacial tension) between two liquids or between a liquid and a solid. Surfactants may act as detergents, wetting agents, emulsifiers, foaming agents, or dispersants.

They are often classified according to the charge of the polar head group:

Anionic Surfactants: These surfactants have a negative charge on their polar head group. Common examples include soap, sodium laureth sulfate, and sodium lauryl sulfate. They are commonly used in detergents and shampoos due to their ability to emulsify oils and hold dirt in suspension, so it can be rinsed away.

Cationic Surfactants: These surfactants have a positive charge on their polar head group. Examples include cetyltrimethylammonium bromide (CTAB) and benzalkonium chloride. These are often used as antiseptics and can also be found in hair conditioners because they reduce static cling.

Nonionic Surfactants: These surfactants have no charge on their polar head group. Examples include alcohol ethoxylates, nonylphenol ethoxylates, and polysorbates. Nonionic surfactants are often used in laundry and dishwasher detergents.

Hydrotropic Surfactants: While not a category of charge like the others, hydrotropic surfactants are a distinct class that promote the solubility of other solutes in water. Sodium xylene sulfonate and sodium cumene sulfonate are examples of hydrotropes.

The selection of a specific type of surfactant depends on the specific application and the properties of the surfactant. Some surfactants can be aggressive and irritating to skin or eyes (like certain anionic surfactants), while others are milder. Similarly, some are better at emulsifying oil or suspending dirt, while others might provide good foaming action or work better in hard water.

All compounds can be retained, separated, and analyzed using a reverse-phase Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Ammonium formate, which serves as a buffer. This analytical method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzalkonium chloride, Cetylpyridinium Chloride, 1-Pentanesulfonic acid, Dodecanoic acid (Lauric acid), p-Toluenesulfonic Acid (PTSA), 1-Octanesulfonic acid, Sodium dodecyl sulfate, 1-Ethylpyridinium bromide

Condition

ColumnNewcrom BH, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN -40-80%, 10 min
BufferAmmonium formate pH 3.0 – 40 mM
Flow Rate1.0 ml/min
DetectionELSD, 50C

Description

Class of CompoundsSurfactants
Analyzing CompoundsBenzalkonium chloride, Cetylpyridinium Chloride, 1-Pentanesulfonic acid, Dodecanoic acid (Lauric acid), p-Toluenesulfonic Acid (PTSA), 1-Octanesulfonic acid, Sodium dodecyl sulfate, 1-Ethylpyridinium bromide

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
1-Ethylpyridinium bromide
1-Octanesulfonic acid
1-Pentanesulfonic acid
Benzalkonium chloride
Cetylpyridinium Chloride
Dodecanoic acid (Lauric acid)
Sodium dodecyl sulfate
p-Toluenesulfonic Acid (PTSA)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Hydrotopic, Cationic, Nonionic and Anion Surfactants on Newcrom BH Column

July 10, 2023

HPLC Method for Analysis of Hydrotopic, Cationic, Nonionic and Anion Surfactants on Newcrom BH by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Hydrotopic, Cationic, Nonionic and Anion Surfactants on Newcrom BH Column
HPLC Method for Separation of Hydrotopic, Cationic, Nonionic and Anion Surfactants

Surfactants, also known as surface-active agents, are compounds that lower the surface tension (or interfacial tension) between two liquids or between a liquid and a solid. Surfactants may act as detergents, wetting agents, emulsifiers, foaming agents, or dispersants.

They are often classified according to the charge of the polar head group:

Anionic Surfactants: These surfactants have a negative charge on their polar head group. Common examples include soap, sodium laureth sulfate, and sodium lauryl sulfate. They are commonly used in detergents and shampoos due to their ability to emulsify oils and hold dirt in suspension, so it can be rinsed away.

Cationic Surfactants: These surfactants have a positive charge on their polar head group. Examples include cetyltrimethylammonium bromide (CTAB) and benzalkonium chloride. These are often used as antiseptics and can also be found in hair conditioners because they reduce static cling.

Nonionic Surfactants: These surfactants have no charge on their polar head group. Examples include alcohol ethoxylates, nonylphenol ethoxylates, and polysorbates. Nonionic surfactants are often used in laundry and dishwasher detergents.

Hydrotropic Surfactants: While not a category of charge like the others, hydrotropic surfactants are a distinct class that promote the solubility of other solutes in water. Sodium xylene sulfonate and sodium cumene sulfonate are examples of hydrotropes.

The selection of a specific type of surfactant depends on the specific application and the properties of the surfactant. Some surfactants can be aggressive and irritating to skin or eyes (like certain anionic surfactants), while others are milder. Similarly, some are better at emulsifying oil or suspending dirt, while others might provide good foaming action or work better in hard water.

All compounds can be retained, separated, and analyzed using a reverse-phase Newcrom BH, 4.6 x 250 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Ammonium formate, which serves as a buffer. This analytical method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzalkonium chloride, Cetylpyridinium Chloride, Triton X100, 1-Pentanesulfonic acid, 1-Hexanesulfonic acid, sodium salt, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, Sodium dodecyl sulfate, 1-Octanesulfonic acid

Condition

ColumnNewcrom BH, 4.6 x 250 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN -40-80%, 30 min
BufferAmmonium formate pH 3.0 – 20 mM
Flow Rate1.0 ml/min
DetectionELSD, 50C

Description

Class of CompoundsAliphatic sulfonic acid
Analyzing CompoundsBenzalkonium chloride, Cetylpyridinium Chloride, Triton X100, 1-Pentanesulfonic acid, 1-Hexanesulfonic acid, sodium salt, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, Sodium dodecyl sulfate, 1-Octanesulfonic acid

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
1-Decanesulfonic acid
1-Heptanesulfonic acid
1-Hexanesulfonic acid, sodium salt
1-Octanesulfonic acid
1-Pentanesulfonic acid
Benzalkonium chloride
Cetylpyridinium Chloride
Sodium dodecyl sulfate
Triton X100
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Benzalkonium Chloride on Primesep SB Column

February 8, 2023

HPLC Method for Analysis of Benzalkonium chloride on Primesep SB by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

Benzalkonium chloride, also known as Zephiran or Alkyldimethylbenzylammonium chloride, is a positively charged surfactant often used as a pharmaceutical preservative and as an antiseptic. Benzalkonium Chloride can be retained on a mixed-mode stationary phase Primesep SB column with embedded strong basic ion-pairing groups, using a gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) ionic modifier.  This analysis method can be UV detected at 210 nm with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Benzalkonium chloride

Condition

ColumnPrimesep SB, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 30-70%, 10 min
BufferH2SO4 – 0.2%
Flow Rate0.5 ml/min
DetectionUV 210 nm
Peak Retention Time5.32, 6.93

Description

Class of CompoundsDrug
Analyzing CompoundsBenzalkonium chloride

Application Column

Primesep SB

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Benzalkonium chloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Benzalkonium Chloride BCT 8358 on Primesep D column

July 8, 2011

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Benzalkonium chloride is a mixture of alkylbenzyldimethylammonium chlorides of various even-numbered alkyl chain lengths. It is used as a surfactant. Hydrophobic quaternary amines, like BTC, produce poor peak shape in reversed-phase chromatography due to interaction of quaternary amines with residual silanols. Ideal peak shape can be achieved on reversed-phase anion-exchange columns like Primesep D. Primesep D has a basic group on the surface of silica gel that shields silanols from interacting with quaternary, tertiary, secondary and primary amines. This allows to achieve symmetrical peaks for hydrophobic basic analytes. Compounds need to be hydrophobic enough to get compensation for repulsion effect coming from stationary phase. BTC can be monitored by ELSD and UV. Primesep D will also retain acidic counter-ions of quaternary amines, so simultaneous analysis of hydrophobic basic and hydrophilic basic compounds is possible.

Condition

Column Primesep D, 4,6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection 210

 

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Description

Class of Compounds
 Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Benzalkonium Chloride BCT 8358

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Benzalkonium chloride

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Surfactants

November 21, 2006


Surfactants are molecules that contain both hydrophilic and hydrophobic groups, usually in the form of a hydrophilic head and a hydrophobic tail. Surfactants are used in detergents where they can form micelles around hydrophobic dirt molecules and wash them away. Triton X-100 is a surfactant with a hydrophilic polyethylene oxide chain that can be separated on a Primesep D reverse-phase HPLC column based on the number of oxide units in the chain. The mobile phase is water, acetonitrile (MeCN, ACN) and sulfuric acid as buffer. UV detection at 210nm.

Condition

Column Primesep D,  4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV 210nm

 

Description

Class of Compounds
Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Triton X-100, Cetylpyridinium Chloride

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Benzalkonium chloride
Cetylpyridinium Chloride
Sodium
Triton X100

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.