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Separation of Benzonitrile Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds  Primesep 100 separates a mixture of amino acids (tyrosine, phenylalanine), organic acids (benzoic acid, mandelic acid), amines (benzylamine, pyridine), and neutrals (benzonitrile, toluene) in one HPLC run by combining reversed-phase, cation-exchange, and polar interactions. The method is tunable and peak order can be changed significantly by adjusting acetonitrile and trifluoroacetic acid concentrations. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and compatible with UV, mass spec (LC/MS) and evaporative light scattering (ELSD) detection. Application Analytes: Amino Acids Benzoic Acid Benzonitrile Benzylamine Mandelic Acid Phenylalanine Pyridine Toluene Tyrosine Application Detection: UV Detection Neutral, Base, and Acid on Primesep A Column  Primesep A separates acids, bases, and neutrals in one injection. Maleic acid, benzonitrile, and benzylamine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Benzonitrile Benzylamine Maleic Acid Application Detection: UV Detection Neutral, Base, and Acid on Primesep 100 Column  Primesep 100 retains and separates an acid, base, and neutral in one HPLC injection. Maleic acid, benzylamine, and benzonitrile are resolved by ion-exclusion, ion-exhange and reversed-phase modes. The separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Benzonitrile Benzylamine Maleic Acid Application Detection: UV Detection Neutral, Base, and Acid on Primesep 200 Column  Primesep 200 separates acids, bases, and neutrals in one injection. Maleic acid, benzonitrile, and benzylamine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Benzonitrile Benzylamine Maleic Acid Application Detection: UV Detection Neutral, Base, and Acid on Primesep B Column  Primesep B separates acids, bases, and neutrals in one injection. Maleic acid, benzonitrile, and benzylamine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Benzonitrile Benzylamine Maleic Acid Application Detection: UV Detection Acid Effect on Retention of Acidic Analytes
In mixed-mode chromatography, retention time and elution order can be changed for acidic analytes based on the pH of the mobile phase. In this application, the order of elution for benzoic acid and benzonitrile is changed by changing the pH of the mobile phase. At lower pH (pH-2, TFA), ionization of carboxylic acid fragment of benzoic acid is totally suppressed (not ionized), and benzoic acid does not show any anion-exchange properties. As the pH of the mobile phase increases (pH 4, formic acid), the carboxylic acid fragment of benzoic acid is ionized and participates in ion-exchange interaction with positively charged sites of the mixed-mode column. In most cases, changing pH does not change retention of neutral analytes. Mixed-mode chromatography is compatible with all detection techniques (UV, ELSD, CAD, LC/MS, etc.) Application Analytes: Benzoic Acid Benzonitrile Application Detection: UV Detection Effect of mobile phase composition on retention of 3 compounds on Obelisc R
Application Analytes: Benzoic Acid Benzonitrile Benzylamine Application Detection: UV Detection HPLC Separation of Amino Acids, Bases, Acids, and Neutrals on Obelisc R  Application Analytes: Benzoic Acid Benzonitrile Benzylamine Phenol Phenylalanine Pyridine Toluene Trypthophan 2,6-Lutidine Application Detection: UV Detection HPLC Separation of Methyl Paraben, Benzonitrile, Propyl Paraben, and Toluene on Mixed-Mode and Reverse Phase Columns
Application Analytes: Benzonitrile Methyl Paraben Propyl Paraben Toluene Application Detection: UV Detection HPLC Separation of Dequalinium and Benzonitrile on Different Columns  Dequalinium, or Dequadin, is an antiseptic and disinfectant. It consists of two quaternary amines connected by C10 hydrophobic chain. It's a strongly basic compound that is hard to analyze on reverse phase column and obtain good peak shape. Compound shows a significant tailing (symmetry below 0.5 and USP tailing factor of over 2) due to interaction of quaternary groups with residual silanols. In this HPLC application dequalinium is analyzed on Primesep D mixed-mode column with perfect symmetry and high efficiency. Primesep D column has hydrophobic chain and a basic group close to the surface of silica gel. This group provides shielding effect and prevents interaction between acidic silanol groups and quaternary amines of dequalinium. Method can be used for analysis and quantitation of hydrophobic amines when peak shape is not satisfactory on traditional HPLC columns. Relative comparison of several modern LC columns (Zorbax from Agilent, XTerra from Waters and Gemini from Phenomenex) shows that Primesep D mixed-mode column can be successfully used in determination of hydrophobic quaternary amines and diamines. If a compound is retained on C18 column with poor peak shape, switching to mixed-mode column can provide a valuable alternative. Benzonitrile is used to ensure high quality of packing and good peak shape for neutral analytes. Method uses trifluoroacetic acid as an additive to the mobile phase. Other acids and buffers can be used depending on the detection technique. Application Analytes: Dequalinium Benzonitrile Application Detection: UV Detection |
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