HPLC Method for Dimethylglycine, Methylglycine (Sarcosine), Trimethylglycine (Betaine), Glycine on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Dimethylglycine, Methylglycine (Sarcosine), Trimethylglycine (Betaine), Glycine.

Glycine, also written as Gly or G, is an important amino acid compounds widely used in pharmaceutical, biochemical, and peptide research. It has the chemical formula C₂H₅NO₂. It is water-soluble and plays a critical role in protein synthesis, peptide modification, and metabolic studies. It can be found in meat., eggs. and bones. You can find detailed UV spectra of Glycine and information about its various lambda maxima by visiting the following link.

Methylglycine, also known as Sarcosine, is an amino acid with the chemical formula C₃H₇NO₂. It is primarily known as a potential disease marker, as it does not typically occur in urine, but it is an intermediate and byproduct of glycine synthesis and degradation. You can find detailed UV spectra of Methylglydine and information about its various lambda maxima by visiting the following link.

Dimethylglycine is a derivative of glycine with the chemical formula C₄H₉NO₂. While it is found primarily in beans and liver, it is said to have a sweet taste. There has been research done into using Dimethylglycine as treatment for autism, epilepsy, and mitochondrial disease, though no evidence to suggest it’s effectiveness as a treatment was found. You can find detailed UV spectra of Dimethylglycine and information about its various lambda maxima by visiting the following link.

Trimethylglycine, also known as betaine, is a compound derived from the amino acid glycine with the chemical formula C₅H₁₁NO₂. It is found in various foods, including beets, spinach, whole grains, and seafood. Trimethylglycine has been studied for its potential health benefits, and it is commonly used as a dietary supplement. It plays a role in a process called methylation, which is important for various biochemical reactions in the body. Some studies suggest that trimethylglycine may have potential benefits for heart health as it is believed to help lower levels of homocysteine, an amino acid linked to an increased risk of heart disease when present in high concentrations. You can find detailed UV spectra of Trimethylglycine and information about its various lambda maxima by visiting the following link.

Multi-charged molecules, such as oxaloacetate and oxalate, generally have a tendency to exhibit poor peak shape on reverse-phase HPLC columns, where they show significant tailing. Dimethylglycine, Methylglycine (Sarcosine), Trimethylglycine (Betaine), Glycine can be retained with great peak shape on a Newcrom BH mixed-mode column. Oxalic acid can be measured at low UV. Using a Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer, oxaloacetic acid and oxalic acid can be retained, separated, and UV detected at 200 nm with precise resolution.

If you are looking for optimized HPLC method to analyze Trimethylglycine (Betaine) check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for Analysis of Trimethylglycine (Betaine) on Primesep 100 by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Trimethylglycine (Betaine)

Trimethylglycine, also known as betaine, is a compound derived from the amino acid glycine. It is found in various foods, including beets, spinach, whole grains, and seafood. Trimethylglycine has been studied for its potential health benefits, and it is commonly used as a dietary supplement.

Methylation Support: Trimethylglycine plays a role in a process called methylation, which is important for various biochemical reactions in the body. Methylation is involved in the synthesis of neurotransmitters, DNA, and proteins.Heart Health: Some studies suggest that trimethylglycine may have potential benefits for heart health. It is believed to help lower levels of homocysteine, an amino acid linked to an increased risk of heart disease when present in high concentrations.

Exercise Performance: There is some evidence suggesting that trimethylglycine supplementation may enhance exercise performance. It is thought to improve oxygen utilization and reduce fatigue during physical activity.

Liver Health: Trimethylglycine has been investigated for its potential to support liver health. It may help protect the liver from certain forms of damage.

Anti-Inflammatory Properties: Some studies propose that trimethylglycine may have anti-inflammatory effects, which could be beneficial in various health conditions.

Trimethylglycine can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using UV at 190 nm and SIM 118+

HPLC Method for Analysis of Trimethylglycine (Betaine) on Primesep 100 by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Trimethylglycine (Betaine)

Trimethylglycine, also known as betaine, is a compound derived from the amino acid glycine. It is found in various foods, including beets, spinach, whole grains, and seafood. Trimethylglycine has been studied for its potential health benefits, and it is commonly used as a dietary supplement.

Methylation Support: Trimethylglycine plays a role in a process called methylation, which is important for various biochemical reactions in the body. Methylation is involved in the synthesis of neurotransmitters, DNA, and proteins.Heart Health: Some studies suggest that trimethylglycine may have potential benefits for heart health. It is believed to help lower levels of homocysteine, an amino acid linked to an increased risk of heart disease when present in high concentrations.

Exercise Performance: There is some evidence suggesting that trimethylglycine supplementation may enhance exercise performance. It is thought to improve oxygen utilization and reduce fatigue during physical activity.

Liver Health: Trimethylglycine has been investigated for its potential to support liver health. It may help protect the liver from certain forms of damage.

Anti-Inflammatory Properties: Some studies propose that trimethylglycine may have anti-inflammatory effects, which could be beneficial in various health conditions.

Trimethylglycine be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 200 nm

In this HPLC method, betaine and carboxymethylcysteine are separated by HILIC mixed-mode mechanism. Betaine and carboxymethylcysteine are very hydrophilic compounds which are not retained on reverse phase columns. Compounds are separated by combination of HILIC and ion-exchange mechanisms. In HIILIC mode, Obelisc N columns allow operating at lower organic concentration for improved solubility of analytes. Method can be used for quantitative or qualitative analysis of betaine and carboxymethylcysteine using ESLD or LC/MS detection. Betaine in chemistry is any neutral chemical compound with a positively charged cationic functional group such as ammonium ion or phosphonium ion (generally: onium ions) which bears no hydrogen atom and with a negatively charged functional group such as a carboxylate group which may not be adjacent to the cationic site. Historically the term was reserved for tetramethylglycine only.

The retention of the zwitterion betaine on Primesep 200 demonstrates the tunability of Primesep columns. Retention of betaine is altered by acid type, amount of acid in the mobile phase, and the amount of organic solvent. Typical reversed-phase columns do not show this tunability with simple mass spec compatible mobile phases of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).