| CAS Number | 60628-96-8 |
|---|---|
| Molecular Formula | C22H18N2 |
| Molecular Weight | 310.400 |
| InChI Key | OCAPBUJLXMYKEJ-UHFFFAOYSA-N |
| LogP | 4.77 |
| Synonyms |
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Applications:
HPLC Chiral Separation of Bifonazole on Cyralis CM Column
June 17, 2026
HPLC Method for Analysis of Bifonazole on Cyralis CM Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bifonazole.
Bifonazole is an antifungal drug with the chemical formula C22H18N2. It is primarily sold as an ointment that works through inhibiting fungal ergostrol biosynthesis, which enables it to work against dermatophytes.
This application describes a normal-phase chiral HPLC method for the separation of Bifonazole enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.
Bifonazole can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.
Condition
| Column | Cyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Hexane/Ethanol/DEA- 85/15/0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 280 nm |
| Resolution | 2.32 |
Description
| Class of Compounds | Fungicide |
| Analyzing Compounds | Bifonazole |
Application Column
Cyralis CM
Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Analysis of Bifonazole
August 6, 2015
Bifonazole is an imidazole antifungal drug with fungicidal properties active against dermatophytes. Legacy L1 was used to retain Bifonazole by reverse phase mechanism.Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.
| Column | Legacy L1, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeOH – 80% |
| Buffer | TFA – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Paracetamol |
Application Column
Legacy L1
SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.
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