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Separation of Bromide Separation of Quaternary Amines  Primesep C separates a mixture of quaternary amines by a combination of cation exchange, complex formation, and hydrophic interactions. Methyltriethylammonium, tetraethylammonium, and tetramethylammonium cations as well as bromide counter ion are separated on a short 50 mm column. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and triethylamine acetate with evaporative light scattering detection (ELSD). Application Analytes: Bromide Methyltriethylammonium Quaternary Amines Tetraethylammonium Tetramethylammonium Application Detection: ELSD/MS Detection Simultaneous Separation of Cations and Anions  Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium captions by cation exchange. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD). Application Analytes: Bromide Chloride Potassium Sodium Application Detection: ELSD/MS Detection Effect of Concentration of Organic Modifier on Retention of Cation and Anions  Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium cations by cation exchange. The retention can be adjusted by changing the water/acetonitrile ratio in the mobile phase. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate with evaporative light scattering detection (ELSD). Application Analytes: Bromide Chloride Potassium Sodium Application Detection: ELSD/MS Detection Separation and Retention of Dextromethorphan Bromide in Mixed-Mode HPLC: Buffer Concentration Effect  Application Analytes: Bromide Dextromethorphan Application Detection: UV Detection HPLC Separation of Sodium, Chloride, Bromide, and Iodine
Application Analytes: Bromide Chloride Iodine Sodium Application Detection: ELSD/MS Detection HPLC Separation of Potassium, Perchlorate, Methanesulfonic, Chloride, Bromide, and Nitrate Ions on Obelisc N
Application Analytes: Bromide Chloride Methanesulfonic Acid Nitrate Potassium Application Detection: ELSD/MS Detection HPLC Analysis of Components of Ionic Liquids by Mixed-Mode Chromatography  Ionic liquid is an ionic compound which is liquid at room (or close to room) temperature. Most of the ionic liquids are in a dynamic equilibrium where at any time more than 99.99% of the liquid is made up of ionic, rather than molecular, species. Room-temperature ionic liquids consist of bulky cation (for example, substituted imidazolium) compounds. A wide range of anions is used as counter ions in ionic liquids: organic and inorganic anions such as chloride, iodide, tetrafluoroborate, hexafluorophosphate, bistriflimide, triflate, tosylate. Ionic liquids are widely used as solvents in organic reactions. When products are isolated from ionic liquids, they need to be analyzed for residual ionic liquid content. Because both constituents of the ionic liquid are very different in terms of charge and hydrophobic properties, it is impossible to analyze entire ionic liquids by traditional chromatography. An effective and universal method for analysis of ionic liquids is developed on an Obelisc R HPLC column. Components on the ionic liquids are retained based on ionic and hydrophobic interactions. Obelisc R column has both positively and negatively charged ionic groups, making it possible to retain and separate cations and anions of ionic liquids on one column. Method can be used for quantitative of various ionic liquids containing organic and inorganic ions. Retention time of basic component can be effectively adjusted by pH, stronger anionic and hydrophobic counter-ions might require higher buffer concentration. Composition can be monitored by combination of UV and ELSD or by LC/MS. Application Analytes: Bromide p-Toluenesulfonic Acid (PTSA) TFA (Trifluoroacetic Acid) Perchloric Acid 1-Methyl-3-Propylimidazolium Methylsulfonic Acid Ionic Liquid Application Detection: UV Detection ELSD/MS Detection HPLC Analysis of Basic Drugs and Acidic Counter-Ions by Mixed-Mode Chromatography  Majority of the drugs in pharmaceutical industries are administered in a salt form. The presence of two counter-ions very often requires two methods. The nature of these counterparts in drugs can be: inorganic cation and organic acid, inorganic anion and organic base, and organic cation and organic anion. Based on the property of molecules the stoichiometry can be different also. The task of simultaneous quantitation of counter-ions can be achieved by using mixed-mode columns. The general approach for analysis is based on properties of corresponding counter-ions. Hydrophobic basic drugs, like dextromethorphan, verapamil, trimipramine, and corresponding acidic counter-ions (chloride, chlorate, bromide, bromate, perchlorate, maleate, fumarate,tartrate, succinate, phosphate, citrate, benzosulfonate, toleuensulfonate) can be separated and quantitated in the same run on reversed-phase anion-exchange column. Basic hydrophobic drugs are retained by reversed-phase mechanism, and counter-ion are retained by reversed-phase and anion-exchange mechanism. Some polar counter-ions are retained only by anion-exchange mechanism. Retention time and selectivity of HPLC separation of drugs and counter-ions can be achieved by changing amount of acetonitrile and amount of ions in the mobile phase. Detection technique depends on the properties of counter-ions. In case of low or no UV activity, ELSD can be employed if counter-ion forms non-volatile salt wit mobile phase additive (ammonium formate). This HPLC method can be used for simultaneous quantitation of other basic drugs and counter-ions. Presence of two mechanisms of retention allows to control retention times of drug and counter-ion independently, and even change order of elution when necessary. Application Analytes: Citric Acid Bromide Chloride Chlorate Perchlorate Maleic Acid Fumaric Acid Tartaric Acid Succinic Acid Phosphoric Acid Dextromethorphan Verapamil Pyrilamine Benzenesulfonic Acid p-Toluenesulfonic Acid (PTSA) Application Detection: UV Detection ELSD/MS Detection |
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