Cannabidiol (CBD)

Cannabidiol

CAS Number3556-78-3
Molecular FormulaC21H30O2
Molecular Weight314.469
InChI KeyQHMBSVQNZZTUGM-ZWKOTPCHSA-N
LogP6.5
Synonyms
  • cannabidiol
  • (-)-Cannabidiol
  • 13956-29-1
  • (-)-trans-Cannabidiol
  • UNII-19GBJ60SN5
  • (-)-trans-2-p-Mentha-1,8-dien-3-yl-5-pentylresorcinol
  • delta1(2)-trans-Cannabidiol
  • (-)-CBD
  • GWP42003-P
  • CBD
  • 19GBJ60SN5
  • CHEMBL190461
  • CHEBI:69478
  • QHMBSVQNZZTUGM-ZWKOTPCHSA-N
  • Epidiolex
  • 1,3-Benzenediol, 2-(3-methyl-6-(1-methylethenyl)-2-cyclohexen-1-yl)-5-pentyl-, (1R-trans)-
  • 2-[(1R,6R)-3-methyl-6-prop-1-en-2-ylcyclohex-2-en-1-yl]-5-pentylbenzene-1,3-diol
  • (1'R,2'R)-5'-methyl-4-pentyl-2'-(prop-1-en-2-yl)-1',2',3',4'-tetrahydrobiphenyl-2,6-diol
  • Resorcinol, 2-p-mentha-1,8-dien-3-yl-5-pentyl-, (-)-(E)-
  • Cannabidiol [USAN]

Applications:

HPLC Determination of CBD, CBN, THC, 8-THC and THCA on Cannsep C Column

June 14, 2021

Separation type: Liquid Chromatography Reverse Phase.

HPLC Method for Analysis of Cannabidiol (CBD), Cannabinol (CBN), Tetrahydrocannabinol (THC), Delta-8-Tetrahydrocannabinol (8-THC), Delta-9-Tetrahydrocannabinol (THC), Tetrahydrocannabinolic acid (THCA-A) on Cannsep C by SIELC Technologies

HPLC Determination of CBD, CBN, THC, 8-THC and THCA on Cannsep C Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, THC, 8-THC and THCA.

Δ8-THC, Δ9-THC, CBD, THCA and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a reverse-phase Cannsep C column using a mobile phase consisting of methanol (MeOH), Acetonitrile (MeCN, ACN), and water with sulfuric acid (H2SO4) as buffer and UV detected at 210nm.



ColumnCannsep C, 2.1 x 100 mm, 3 µm, 100 A
Mobile PhaseMeCN/MeOH/H2O – 45/40/15%
BufferH2SO4 – 0.2%
Flow Rate0.2 ml/min
DetectionUV, 200 nm
Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), CBD, CBN, Δ9-THC, Δ8-THC, Tetrahydrocannabinolic acid  (THCA)

Application Column

Cannsep C

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A

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Application Analytes:
Cannabidiol (CBD)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of CBD, CBN, THC in 1:1 Citrus Lime Tonic on Canncep C Column

May 3, 2021

Separation type: Liquid Chromatography Mixed-mode

HPLC Determination of CBD, CBN, THC in 1:1 Citrus Lime (100mg CBD/100mg THC) Tonic on Cannsep C Column
HPLC Determination of CBD, CBN, THC in 1:1 Citrus Lime (100mg CBD/100mg THC) Tonic on Cannsep C Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, delta-9 THC, delta-8 THC


Cannabidiol (CBD), cannabinol (CBN), and delta-9-Tetrahydrocannabinol (THC) are three of the most active cannabinoids in cannabis. THC is the strongest of the three and is the main cause of the psychoactive effects of cannabis. CBD, while the 2nd most active component, does not cause the same effects by itself, but studies show it may have other neurologic and psychiatric benefits. There has been a lot less research conducted on CBN and its potential health benefits, but it is a product of the breakdown of THC and works on the same receptors (albeit with different effects) as CBD.
These cannabinoids can be detected in the low UV regime. Using a Cannsep C reverse-phase column and a mobile phase consisting of water, methanol (MeOH) and acetonitrile (MeCN) with a sulfuric acid (H2SO4) buffer, CBD, CBN, and THC can be separated, measured, and analyzed. This analysis method can be UV detected at 210 nm with high resolution, even in real-world samples.



ColumnCannsep C, 2.1×100 mm, 5 µm, 100A
Mobile PhaseMeCN/MeOH/H2O – 40/35/25%
BufferNo
Flow Rate0.5 ml/min
DetectionUV, 210 nm
Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC),  Cannabinol (CBN)

Application Column

Cannsep C

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabidiol (CBD)
Cannabinol (CBN)
Delta-9-Tetrahydrocannabinol (THC)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of CBD, CBN, delta-9 THC, delta-8 THC on Cannsep C Column

April 30, 2021

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, delta-9 THC, delta-8 THC

Cannabidiol (CBD), cannabinol (CBN), and delta-9-Tetrahydrocannabinol (delta-9 THC) are three of the most active cannabinoids in cannabis. Delta-8-tetrahydrobannabinol also occurs naturally, but in such small amounts that it is often cheaper and easier to synthesize it in the laboratory from CBD. Both forms of THC are the strongest of the cannabinoids and are the main cause of the psychoactive effects of cannabis. CBD, while the 2nd most active component, does not cause the same effects by itself, but studies show it may have other neurologic and psychiatric benefits. There has been a lot less research conducted on CBN and its potential health benefits, but it is a product of the breakdown of THC and works on the same receptors (albeit with different effects) as CBD.
These cannabinoids can be detected in the low UV regime. Using a Cannsep C reverse-phase column and a mobile phase consisting of water and methanol (MeOH) with no buffer, CBD, CBN, delta-9 THC, and delta-8 THC can be separated, measured, and analyzed. This analysis method can be UV detected at 275 nm with high resolution.

Condition

Column Cannsep C, 2.1×100 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 82/18%
Buffer No
Flow Rate 0.5 ml/min
Detection UV, 275 nm

 

Description

Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds  Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC),  Cannabinol (CBN), Delta-8-Tetrahydrocannabinol (THC),

 

Application Column

Cannsep C

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabidiol (CBD)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Δ8-THC, CBD, Δ9-THC, CBN, on Primesep N Column

May 15, 2020


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Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a normal-phase Primesep N column using a mobile phase consisting of hexane and ethyl acetate without the need for a buffer with UV detection at 275nm.

Condition

Column Primesep N , 3.2×150 mm, 3 µm, 100A
Mobile Phase Hexane/Ethyl Acetate – 97/3%
Buffer No
Flow Rate 1.0 ml/min
Detection UV, 275 nm

 

Description

Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds  Delta -8-Tetrahydrocannabinol, Δ8-THC, Cannabidiol (CBD), Delta-9-Tetrahydrocannabinol, Δ9-THC, Cannabinol (CBN)

 

Application Column

Primesep N

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Cannabidiol (CBD)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of CBD, CBN, Δ9-THC, Δ8-THC on Cannsep C Column

May 14, 2020


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Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a reverse-phase Cannsep C column using a mobile phase consisting of methanol (MeOH) and water with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.

Condition

Column Cannsep C, 3.2×150 mm, 3 µm, 100A
Mobile Phase MeOH -80%,
Buffer H2SO4 – 0.2%
Flow Rate 0.5 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds  Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), CBD, CBN, Δ9-THC, Δ8-THC

 

Application Column

Cannsep C

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabidiol (CBD)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of THC in THC Infused Gummy Bear on Cannsep C Column

January 24, 2020

Condition

Column Cannsep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 60-80%, 25 min, 5 min hold
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)

 

Application Column

Cannsep C

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabidiol (CBD)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Ten Cannabinoids on Cannsep C Column

January 13, 2020

Condition

Column Cannsep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 60-80% , 20 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)

 

Application Column

Cannsep C

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabichromene (CBC)
Cannabidiol (CBD)
Cannabidiolic acid (CBDA)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Tetrahydrocannabivarin (THCV)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Ten Cannabinoids on Cannsep B Column

January 13, 2020

Condition

Column Cannsep B, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 50-70% , 25 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)

 

Application Column

Cannsep B

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabichromene (CBC)
Cannabidiol (CBD)
Cannabidiolic acid (CBDA)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Tetrahydrocannabivarin (THCV)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Ten Cannabinoids on Cannsep A Column

January 13, 2020

Condition

Column Cannsep A, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 50-70% , 20 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)

 

Application Column

Cannsep A

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabidiol (CBD)
Cannabidiolic acid (CBDA)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Tetrahydrocannabivarin (THCV)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Fast Separation of Cannabinoids on Mixed-Mode HPLC Column Cannsep B

December 5, 2013

With legalization of marijuana in much of the United States, more and more attention is paid to developing quick, robust and reliable method for quantitation of cannabinoids in marijuana plants and related edible products. The main challenge lies in sample preparation and quantitative analysis of four major cannabinoids: cannabidiol, cannabinol, tetrahydrocannabinol and tetrahydrocannabinolic acid. All compounds are hydrophobic with only THCA (THC-A) and cannabidiolic acid (CBDA) being an acid. Hydrophobic interaction is the main interaction in the separation of cannabinoids on reverse phase columns. On our mixed mode columns acidic molecules retained by ion-exchange mechanism  in addition to hydrophobic. Method can be used for analysis and prep separation of cannabinoids in marijuana plants, seeds and other cannabis-based products. Extraction of cannabinoids is the main procedure in sample preparation. Extraction can be done with organic solvents like chloroform, methanol, ethanol, acetonitrile.  Cannabinoids can be monitored by UV and LC/MS. Mixed-mode columns containing polar embedded groups provide different selectivity than regular reversed-phase columns.

 

Condition

Column Cannsep B, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 40-60% , 20 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)

 

Application Column

Cannsep B

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabichromene (CBC)
Cannabidiol (CBD)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.