Compounds  →  Chloramphenicol

Separation of Chloramphenicol

HPLC Separation of Common Antibiotics in Fish Farming



Fish antibiotics are used in fish farming to treat bacterial diseases of fish. It is common practice in the fish industry, particularly in developing countries, to use large amounts of antibiotics to prevent infection. The antibiotics used are often non-biodegradable and remain in the environment for long periods of time, contaminating soil and ground waters. In farming antibiotics are mixed with food and residual amount of drugs ends up in fish products and poultry, this lead to consumption of antibiotics and metabolites by humans. Five common antibiotics are separated on Primesep C column using LC/MS compatible conditions. Method can be used for quantitative determination of nitrofurantoin, nitrofurazone, furazolidone, furaltadone, chloramphenicol and nitrofurazone in fish products and environment.

Application Analytes:

Nitrofurantoin
Nitrofurazone
Furazolidone
Furaltodone
Chloramphenicol
5-Nitro-2-Furaldehyde Semicarbazone (Nitrofurazone)

Application Detection:

UV Detection

HPLC Separation of Antibiotics in Fish Production



Five antibiotics are separated on Primesep C mixed-mode cation-exchange column using UV, ESLD and LC/MS compatible conditions. Similar compounds can be separated based on reverse phase and cation-exchange mechanisms. Current method can be used in quantitative determination of antibiotics in various food products.

Application Analytes:

Nitrofurantoin
5-Nitro-2-Furaldehyde Semicarbazone (Nitrofurazone)
Furazolidone
Furaltadone
Chloramphenicol

Application Detection:

UV Detection

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