Citric Acid


Citric Acid structural formula

Acids separation with Obelisc N2

CAS Number77-92-9
Molecular FormulaC6H8O7
Molecular Weight192.123
InChI KeyKRKNYBCHXYNGOX-UHFFFAOYSA-N
LogP-1.64
Synonyms
  • Citric acid
  • 2-Hydroxypropane-1,2,3-tricarboxylic acid
  • 1,2,3-Propanetricarboxylic acid, 2-hydroxy-
  • 77-92-9
  • 1,2,3-Propanetricarboxylic acid, 2-hydroxy-
  • 4-03-00-01272
  • 2-Hydroxy-1,2,3-propanetricarboxyic acid
  • 2-Hydroxy-1,2,3-propanetricarboxylic acid
  • 2-Hydroxypropan-1,2,3-tricarboxylic acid
  • 3-Carboxy-3-hydroxypentane-1,5-dioic acid
  • Acide citrique
  • acido citrico
  • Aciletten
  • Celenex 3P6
  • Chemfill
  • Citretten
  • CITRIC ACID ANHYDROUS
  • CITRIC-ACID, ANHYDRIDE CRISTO
  • CITRONENSAEURE
  • Hydrocerol A
  • NSC 112226
  • NSC 30279
  • NSC 626579
  • Propane-1,2,3-tricarboxylic acid, 2-hydroxy-
  • Uro-trainer
  • Zitronensaure
  • BRN 0782061
  • Caswell No. 221C
  • Citric acid, anhydrous
  • EINECS 201-069-1
  • EPA Pesticide Chemical Code 021801
  • FEMA No. 2306
  • FEMA Number 2306
  • 2-Hydroxypropanetricarboxylic acid
  • 2-Hydroxytricarballylic acid
  • Kyselina 2-hydroxy-1,2,3-propantrikarbonova
  • UNII-XF417D3PSL
  • 2-Hydroxy-1,2,3-propanetricarboxylate
  • 2-hydroxypropane-1,2,3-tricarboxylic acid
  • 3-Carboxy-3-hydroxypentane-1,5-dioate
  • Anhydrous citrate
  • Anhydrous citric acid
  • Citraclean
  • Citrate
  • Citro
  • E 330
  • E330
  • H3cit
  • Kyselina citronova
  • Suby G
  • beta-Hydroxytricarballylate
  • beta-Hydroxytricarballylic acid
  • 12262-73-6
  • 43136-35-2
  • 136108-93-5
  • 245654-34-6
  • 623158-96-3
  • 856568-15-5
  • 878903-72-1
  • 890704-54-8
  • 896506-46-0
  • 906507-37-7
  • 1192555-95-5
  • 2023788-69-2

Applications:

HPLC Method for Analysis of Citric Acid and Potassium Bromide on Newcrom BH Column

August 8, 2022

Separation type: Liquid Chromatography Mixed-mode
 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Citric Acid and Potassium Bromide

 

Citric Acid is a naturally ocurring organic acid found in citrus fruits; it is also an intermediate in the citric acid cycle of aerobic organisms. It is used industrially as an acidity regulator, flavoring, detergent, and more than 2 million tons are produced annually. Potassium Bromide used to be a popular anticonvulsant and sedative, and is now mainly used as an antiepileptic medication for dogs. Citric acid and Potassium Bromide can be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Trifluoroacetic acid (TFA). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry. This method is also compatible with Mass Spectrometry (MS), ELSD, and CAD. The Citric acid was detected via negative Electrospray Ionization (ESI-) at an m/z of 191.

Condition

Column Newcrom BH, 3.2×100 mm, 3 µm, 100A
Mobile Phase MeCN – 10%
Buffer TFA – 0.1%
Flow Rate 0.5 ml/min
Detection UV 210 nm, ESI

 

Description

Class of Compounds
Acid
Analyzing Compounds Citric Acid, Potassium Bromide

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Bromide
Citric Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Tartaric Acid and Citric Acids on Newcrom BH Column

March 22, 2021


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Condition

Column Newcrom BH, 4.6×150 mm, 3 µm, 100A
Mobile Phase H2O
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV 200 nm

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds Tartaric Acid, Citric Acid

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Citric Acid
Tartaric Acid
dl-Tartaric acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Citric Acid on Newcrom B Column

October 24, 2019

HPLC Determination of Citric Acid on Newcrom B Column

 

Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer Formic Acid – 0.5%
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Citric Acid

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Citric Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Small Organic Acids on Newcrom B Column

October 22, 2019


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Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer AmAc pH 5.0, Formic Acid
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Glycolic acid, Succinic Acid, Methylmalonic acid, Malic Acid, Maleic Acid, Citric Acid, Tartaric Acid, Fumaric Acid, Malonic Acid

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Citric Acid
Fumaric Acid
Gluconic acid
Glycolic acid
Maleic Acid
Malic Acid
Malonic Acid
Methylmalonic Acid
Succinic Acid
Tartaric Acid
dl-Tartaric acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Compounds in TCA Cycle on Primesep D Column

July 3, 2013

The citric acid cycle (tricarboxylic acid cycle, Krebs cycle) is a key process in the metabolic pathway by which all aerobic organisms generate energy. Compounds generated during TCA are hydrophilic, acidic compounds. Some of the hydrophilic acids are very strong while others are relatively weaker. All compounds have very low hydrophobicity and do not retain by the reversed-phase mechanism on the C18 or C8 HPLC columns. Citric acid and related products were separated in reversed-phase, anion-exchange mode on the Primesep D mixed-mode column. All hydrophilic, acidic compounds are well separated and demonstrate good shape. This robust method can be used for analysis of components of the TCA cycle and other highly hydrophilic compounds. This method is fully compatible with LC/MS and prep chromatography.

Condition

Column Primesep D, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm, pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Acid, Hydrophilic, Ionizable
Analyzing Compounds Sodium, Succinic acid, Malic acid, Isocitric acid, Citric acid, Fumaric acid, Maleic acid

 

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Citric Acid
Fumaric Acid
Isocitric Acid
Maleic Acid
Malic Acid
Sodium
Succinic Acid

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Analysis of Compounds of TCA Cycle on SHARC 1 Column

July 2, 2013

This is an alternative approach for the separation of the TCA cycle intermediates that was developed using novel hydrogen-bonding HPLC column. The organic nature of the mobile phase helps obtain a highly sensitive LC/MS compatible method.

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

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Application Analytes:
Citric Acid
Fumaric Acid
Isocitric Acid
Maleic Acid
Malic Acid
Succinic Acid

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Barium Cation in the Presence of Citric Acid, Sucrose and Tween 80

July 8, 2011

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Barium ion and citric acid are analyzed in the presence of sucrose and Tween 80 on Obelisc N HILIC mixed-mode column. Both compounds are retained in ion-exchange mode and HILIC is not employed with this method. Obelisc N HPLC column has basic and acidic ion-exchange groups separated by long hydrophilic chain. Presence of that long chain allows both cation- and anion-exchange mechanisms to exist simultaneously. Obelisc N column can be used for analysis of cation and anions in HILIC/ion-exchange mode and in ion-exchange mode. Analytes can be monitored by ELSD/CAD and LC/MS.

 

Condition

Column Obelisc N, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc pH 4.0
Flow Rate 1.0 ml/min
Detection ELSD

 

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Description

Class of Compounds
 Acid, Ion,  Hydrophobic, Ionizable
Analyzing Compounds Barium ion, Citric acid,  Sucrose, Tween 80

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Citric Acid
Sucrose
Tween 80
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of Pseudoephedrine and Citric Acid on Primesep Column

October 14, 2010


Pseudoephedrine is a drug used in cough and cold compositions. One of the preservatives in solutions of pseudoephedrine is citric acid. Both compounds are hydrophilic in nature, with pseudoephedrine being a basic compound and citric acid an acidic compound. Separation of these two compounds is achieved on a Primesep N column. Compounds are separated by combination of HILIC, cation-exchange and anion-exclusion mechanisms. Pseudoephedrine and citric acid are monitored by UV. This HPLC method can be adopted as generic approach for analysis of pseudoephedrine and other hydrophilic drugs and preservatives in mixtures.

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep N

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Citric Acid
Pseudoephedrine (PSE)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Citric Acid and Phosphate Ions in HILIC Chromatography

July 16, 2009

Citric acid and phosphate ion are separated by HILIC on Primesep N HILIC HPLC column. Both compounds are very hydrophilic. Citric acid and phosphate ions are well separated with excellent peak shape. Method can be used for quantitation of citrate and phosphate in various formulations. Because of the lack of UV activity, separation can be monitored by LC/MS, ELSD or Corona CAD. Method can be used for other hydrophilic organic and inorganic acids.

Application Column

Primesep N

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Citric Acid
Phosphate

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of Basic Drugs and Acidic Counter-Ions by Mixed-Mode Chromatography

July 16, 2009

The majority of drugs in the pharmaceutical industry are administered in salt form. The presence of two counter-ions very often necessitates the use of two methods. The nature of these counterparts in drugs can be an inorganic cation and organic acid, inorganic anion and organic base, and organic cation and organic anion. Furthermore, the properties of the molecules will result in a differing stoichiometry. The task of simultaneous quantitation of counter-ions can be achieved by using mixed-mode columns. The general approach for analysis is based on properties of corresponding counter-ions. Hydrophobic basic drugs, like dextromethorphan, verapamil, trimipramine, and corresponding acidic counter-ions (chloride, chlorate, bromide, bromate, perchlorate, maleate, fumarate,tartrate, succinate, phosphate, citrate, benzosulfonate, toleuensulfonate) can be separated and quantitated in the same run on reversed-phase anion-exchange column. Basic hydrophobic drugs are retained by the reversed-phase mechanism, and counter-ions are retained by the reversed-phase and anion-exchange mechanism. Some polar counter-ions are retained only by the anion-exchange mechanism. Retention time and selectivity of HPLC separation of drugs and counter-ions can be achieved by changing the amount of acetonitrile and the amount of ions in the mobile phase. The detection technique depends on the properties of the counter-ions. In case of low or no UV activity, ELSD can be employed if the counter-ion forms a non-volatile salt with the mobile phase additive (ammonium formate). This HPLC method can be used for simultaneous quantitation of other basic drugs and counter-ions. The presence of two mechanisms of retention allows control over retention times of drug and counter-ion independently, and even allows a change of order of elution when necessary.

Condition

Column Primesep D , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD, UV 270

Description

Class of Compounds Ions, Hydrophilic, Hydrophobic, Base, Acids, Ionizable
Analyzing Compounds Sodium Chloride, Sodium chloride, Sodium Chlorate, Sodium bromide, Sodium bromate, Perchloric Acid, Maleic Acid, Fumaric Acid, Tartaric Acid, Succinic Acid, Phosphoric Acid, Citric acid, Benzosulfonic acid,  Dextromethorphan, Verapamil, Trimipramine

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Benzenesulfonic Acid
Bromide
Chlorate
Chloride
Citric Acid
Dextromethorphan
Fumaric Acid
Maleic Acid
Organic Acids
Perchlorate
Phosphoric Acid
Pyrilamine
Succinic Acid
Tartaric Acid
Verapamil
p-Toluenesulfonic Acid (PTSA)

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Alginic Acid and Related Products

August 22, 2008

Alginate is used in various pharmaceutical preparations. Chemically, it is a linear copolymer with homopolymeric blocks of (1-4)-linked ?-D-mannuronate (M) and its C-5 epimer ?-L-guluronate (G) residues, respectively, covalently linked together in different sequences or blocks. Alginic acid can be separated from benzoate, citric acid and saccharin by mixed-mode chromatography on Primesep C HPLC column. This method can be used to quantitate alginic acid, citric acid or saccharin in complex mixtures. Various detection technique can be used (UV, ELSD, LC/MS), based on mobile phase selection.

Condition

Column Primesep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Na2HPO4
Flow Rate 0.25 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Acid, Hydrophilic, Ionizable
Analyzing Compounds Alginic acid, Citric acid, Benzoic acid, Saccharin

 

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Alginic Acid
Benzoic Acid
Citric Acid
Saccharin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.