| CAS Number | 372-75-8 |
|---|---|
| Molecular Formula | C6H13N3O3 |
| Molecular Weight | 175.188 |
| InChI Key | RHGKLRLOHDJJDR-BYPYZUCNSA-N |
| LogP | -3.13 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of L-Citrulline
January 9, 2026
If you are looking for optimized HPLC method to analyze Citrulline check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Citrulline on Primesep 100 Column
January 8, 2026
HPLC Method for Citrulline on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Citrulline.
Citrulline is an alpha-amino acid with the chemical formula C6H13N3O3. It is a key intermediate in the urea cycle. It can be derived from a variety of sources, such as arginine, ornithine, and asymmetric dimethylarginine. Commercially, it is used as a dietary supplemant as L-citrulline boots the production of nitric oxide, which helps with arterial function.
Citrulline can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | H2SO4 – 0.1% |
| Buffer | MeCN/H2O – 10/90% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |
| LOD* | 0.3 ppm |
| Class of Compounds | Amino Acid |
| Analyzing Compounds | Citrulline |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Analysis of Citrulline and Arginine on Primesep Columns
October 14, 2010
Two underivatized amino acids, citrulline and arginine, are separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns with LC/MS compatible mobile phase. Method can be used for retention and separation of underivatized amino acids without ion-pairing reagent in the mobile phase.
| Column | Primesep, 2.1×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm pH 3.0 |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD |
| Class of Compounds |
Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Citrulline, Arginine, Chloride |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsCitrulline
HPLC Separation of Citrulline and Arginine on Primesep 200 Column
November 21, 2006
Citrulline and Arginine are α-amino acids that differ only in one functional group. They can be separated on a reverse-phase Primesep 200 HPLC column by hydrophobic and cation-exchange mechanisms in a mobile phase of water and acetonitrile (MeCN, ACN) with a gradient buffer of formic acid. Evaporative Light Scattering Detector (ELSD) used.
| Column | Primesep 200, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Citrulline, Arginine |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsCitrulline
Sodium
