 |
|
 |
|
|
Separation of Cysteine Amino Acids Analysis in Acid Gradient Condition  11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD). Application Analytes: Alanine Amino Acids Arginine Aspartic Acid Cysteine Glutamic Acid Histidine Isoleucine Lysine Methionine Phenylalanine Valine Application Detection: UV Detection Hydrophobic and Hydrophilic Compound Separation  Primesep 100 separates a mixture of polar and nonpolar compounds in one analytical run. The amino acid cysteine; amino acid derivatives L-cystine, 2,2-dimethylcystine, and 2-methylcysteine; the polar acid benzoic acid; and the nonpolar neutral toluene are separated by a gradient using a combination of polar and hydrophobic interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 210 nm. Application Analytes: Amino Acids Benzoic Acid Cysteine L-Cystine 2,2-Dimethylcystine 2-Methylcysteine Toluene Application Detection: UV Detection |
|
SIELC Technologies | 65 East Palatine Road, Suite 221, Prospect Heights, IL USA 60070 | Tel: (847) 229-2629 Fax: (847) 655-6079 | Email: mail@sielc.com | sitemap
Copyright © SIELC Technologies. 2002-2012 |