Cysteine

Cysteine

Synonyms

    Applications:

    HPLC Method for Separation of Sulfur-containing Biomolecules on Primesep 100  Column

    June 13, 2023

    HPLC Method for Separation of Cysteine, Glutathione, reduced, Cystine, Cysteine-glutathione disulfide, Glutathione oxidized on Primesep 100 by SIELC Technologies

    Separation type: Liquid Chromatography Mixed-mode

    HPLC Method for Separation of Cysteine, Glutathione, reduced, Cystine, Cysteine-glutathione disulfide, Glutathione oxidized on Primesep 100 Column by SIELC Technologies
    HPLC Method for Separation of Cysteine, Glutathione, reduced, Cystine, Cysteine-glutathione disulfide, Glutathione oxidized on Primesep 100 Column by SIELC Technologies

    These compounds are all involved in redox reactions and cellular defenses against oxidative stress in biological systems. Here’s a bit more about each of them:

    1. Cysteine (Cys): This is a sulfur-containing amino acid that’s used in the biosynthesis of proteins. Its thiol side chain often participates in enzymatic reactions, and contributes to the stability of proteins by forming disulfide bonds.
    2. Glutathione, reduced (GSH): A tripeptide (small protein) consisting of the amino acids glutamic acid, cysteine, and glycine. GSH serves as an antioxidant, helping to prevent damage to cellular components caused by reactive oxygen species such as free radicals and peroxides.
    3. Cystine: This is a covalently bonded dimer molecule of two cysteine molecules, connected through a disulfide bond. Disulfide bonds between cysteine residues in peptide chains contribute to the 3D structure of proteins. In dietary supplements and food labeling, this compound is often referred to as a conditionally essential amino acid.
    4. Cysteine-glutathione disulfide (CySSG): This is a mixed disulfide formed by the reaction of glutathione with the oxidized form of cysteine (cystine). It is one of the forms in which cysteine is stored and transported in plasma.
    5. Glutathione, oxidized (GSSG): This is the oxidized form of glutathione, produced when two glutathione molecules are linked by a disulfide bond. GSSG is reduced back to GSH by the enzyme glutathione reductase, which also requires NADPH as a cofactor.

    Overall, these molecules play vital roles in the body’s antioxidant defenses, detoxification of xenobiotics, modulation of redox-controlled signaling pathways, and regulation of cellular proliferation and apoptosis.

    These compounds can be retained, separated, and analyzed using a reverse-phase Primesep 100, 4.6 x 150 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Sulfuric acid, which serves as a buffer. This analytical method can be

    High Performance Liquid Chromatography (HPLC) Method for Analysis of Cysteine, Cystine, Cysteine-glutathione disulfide, L-Cysteine

    Condition

    ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
    Mobile PhaseMeCN -10%
    BufferGradient H2SO4 0.1-0.3%, 10 min
    Flow Rate1.0 ml/min
    DetectionUV 200 nm

    Description

    Class of CompoundsThiol, Amino acid
    Analyzing CompoundsCysteine, Glutathione, reduced, Cystine, Cysteine-glutathione disulfide, Glutathione oxidized

    Application Column

    Primesep 100

    Column Diameter: 4.6 mm
    Column Length: 150 mm
    Particle Size: 5 µm
    Pore Size: 100 A

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    Application Analytes:
    Cysteine
    Cysteine-glutathione disulfide
    Cystine
    L-Cysteine

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Method For Analysis Of Cysteine and Cystine on Primesep 100 Column

    March 10, 2022

    Separation type: Liquid Chromatography Mixed-mode

    HPLC Method For Analysis Of Cysteine and Cystine on Primesep 100 Column

    High Performance Liquid Chromatography (HPLC) Method for Analysis of Cysteine and Cystine

    Cysteine is an amino acid that is an essential building block of a wide variety of proteins made and used throughout the entire body. Cystine is an oxidized dimer of cysteine that the body uses for redox reactions and as a linkage for proteins to keep their 3D structures. Cysteine and Cystine can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.

    ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
    Mobile PhaseMeCN/H2O – 20/80%
    BufferH2SO4 – 0.1%
    Flow Rate1.0 ml/min
    DetectionUV, 200 nm
    Class of Compounds
    Amino Acid
    Analyzing CompoundsCysteine,  Cystine

    Application Column

    Primesep 100

    Column Diameter: 4.6 mm
    Column Length: 150 mm
    Particle Size: 5 µm
    Pore Size: 100 A

    Add to cart
    Application Analytes:
    Cysteine
    Cystine
    L-Cysteine

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of Mixture of 12 Amino Acids on Primesep 100 Column

    March 11, 2019

     

    Condition

    Column Primesep 100, 4.6×250 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 35/65%
    Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
    Flow Rate 1.0 ml/min
    Detection UV, 200 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds L-Asparagine (Asn/N), L-Threonine (Thr/T), L-Cysteine (Cys/C), L-Proline (Pro/P), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L- Tryptophan (Trp/W), L-Histidine (His/H), L-Arginine (Arg/R)

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Amino Acids
    Arginine
    Asparagine
    Cysteine
    D-Isoleucine
    D-Leucine
    D-Valine
    DL-Isoleucine
    Histidine
    Isoleucine
    L-Cysteine
    L-Methionine
    L-Threonine
    Leucine
    Methionine
    Phenylalanine
    Proline
    Tryptophan
    Valine
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of Mixture of Conditionally Essential Amino Acids on Primesep 100 Column

    March 11, 2019

     

    Condition

    Column Primesep 100, 4.6×250 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 35/65%
    Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
    Flow Rate 1.0 ml/min
    Detection UV, 200 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds Glutamin (Gln/Q), L-Glycine (Gly/G), L-Cysteine (Cys/C), L-Proline Pro/p), L-Tyrosine (Tyr/Y), L-Arginine (Arg/R)

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Amino Acids
    Arginine
    Cysteine
    Glycine
    L-Cysteine
    L-Glutamine
    Proline
    Tyrosine
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Amino Acids Analysis in Acid Gradient Condition

    September 18, 2006

    11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).

    Condition

    Column Primesep 100, 4.6×250 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 30/70%
    Buffer TFA , gradient  0.05-0.3 % , 25 min
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Aspartic acid, Glutamic acid, Alanine, Valine, Methionine, Isoleucine, Cysteine, Phenylalanine, Histidine, Lysine, Arginine

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Alanine
    Amino Acids
    Arginine
    Aspartic Acid
    Cysteine
    Glutamic Acid
    Histidine
    Isoleucine
    Lysine
    Methionine
    Phenylalanine
    Valine

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Hydrophobic and Hydrophilic Compound Separation

    September 25, 2003

    Primesep 100 separates a mixture of polar and nonpolar compounds in one analytical run. The amino acid cysteine; amino acid derivatives L-cystine, 2,2-dimethylcystine, and 2-methylcysteine; the polar acid benzoic acid; and the nonpolar neutral toluene are separated by a gradient using a combination of polar and hydrophobic interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 210 nm.

    Condition

    Column Primesep 100, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer H2SO4
    Flow Rate 1.0 ml/min
    Detection UV 210 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Nonpolar, Polar, Supplements
    Analyzing Compounds Cysteine,  L-cystine, 2,2-dimethylcystine,  2-methylcysteine, Benzoic Acid,  Toluene

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    2,2-Dimethylcysteine
    2-Methylcysteine
    Amino Acids
    Benzoic Acid
    Cysteine
    L-Cystine
    Toluene

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.