HPLC Method for Cordycepin, Deoxyadenosine on BIST B+ by SIELC Technologies

Cordycepin is an adenosine analogue with the chemical formula C₁₀H₁₃N₅O₃. It is reported to prevent cell reproduction in various cancer cells. It also might possess antioxidant and anti-inflammatory properties, when considered in addition with it’s ability to cross the blood-brain barrier, it may become widely used in pharmaceuticals.

Deoxyadenosine is a deoxyribonucleoside with the chemical formula C₁₀H₁₃N₅O₃. It is a derivative of adenosine. High presence of it can kill T lymphocytes and kill those cells, leading to adenosine deaminase severe combined immunodeficiency disease, also known as ADA-SCID.

Cordycepin, Deoxyadenosine can be retained and analyzed using the BIST B+ stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Phosphoric Acid buffer. Detection is performed using UV.

Description

HPLC Method for Separation of Adenine, Deoxyadenosine, Adenosine on BIST B+ by SIELC Technologies.

Adenine, also noted as A and Ade, has the chemical formula C₅H₅N₅. Besides DNA and RNA, Adenine can also be found in Adenosine triphosphate (ATP), which is a nucleotide triphosphate that provides energy in cells required for bodily functions. In DNA, it partners with Thymine via two hydrogen bonds, while in RNA it bonds to Uracil for protein synthesis.

Deoxyadenosine is a deoxyribonucleoside with the chemical formula C₁₀H₁₃N₅O₃. It is a derivative of adenosine. High presence of it can kill T lymphocytes and kill those cells, leading to adenosine deaminase severe combined immunodeficiency disease, also known as ADA-SCID.

Adenosine is a key building block of energy-carrying molecules with the chemical formula C₁₀H₁₃N₅O₄. It has a variety of other uses, including being a inhibitory neurotransmitter which helps with sleep and acting as a blood flow regulator. Medicinally, it is used as treatment for supraventricular tachycardia (SVT). You can find detailed UV spectra of Adenosine and information about its various lambda maxima by visiting the following link.

Adenine, Deoxyadenosine, Adenosine can be retained and analyzed using the BIST B+ stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Description

HPLC Method for Deoxyadenosine, Adenosine on Newcrom AH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method of Adenosine and Deoxyadenosine.

Adenosine is a key building block of energy-carrying molecules with the chemical formula C₁₀H₁₃N₅O₄. It has a variety of other uses, including being a inhibitory neurotransmitter which helps with sleep and acting as a blood flow regulator. Medicinally, it is used as treatment for supraventricular tachycardia (SVT). You can find detailed UV spectra of Adenosine and information about its various lambda maxima by visiting the following link.

Deoxyadenosine is a deoxyribonucleoside with the chemical formula C₁₀H₁₃N₅O₃. It is a derivative of adenosine. High presence of it can kill T lymphocytes and kill those cells, leading to adenosine deaminase severe combined immunodeficiency disease, also known as ADA-SCID.

Deoxyadenosine, Adenosine are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: adenosine and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and ammonium formate (AmFm) buffer. Detection can be achieved with UV 260 nm, mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (CAD).

HPLC Method for Adenosine, Guanosine, Deoxyguanosine, Deoxyadenosine on Newcrom AH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Adenosine, Guanosine, Deoxyguanosine, Deoxyadenosine.

Guanosine is a purine nucleoside with the chemical formula C₁₀H₁₃N₅O₅. It can be phosphorylated into many other forms, which play vital roles in biochemical possesses like synthesis of nucleic acids, proteins, photosynthesis, and more. It is also required for RNA splicing.

Deoxyguanosine is a deoxyribonucleoside with the chemical formula C₁₀H₁₃N₅O₄. It is a vital part of what makes up DNA.

Adenosine is a key building block of energy-carrying molecules with the chemical formula C₁₀H₁₃N₅O₄. It has a variety of other uses, including being a inhibitory neurotransmitter which helps with sleep and acting as a blood flow regulator. Medicinally, it is used as treatment for supraventricular tachycardia (SVT). You can find detailed UV spectra of Adenosine and information about its various lambda maxima by visiting the following link.

Deoxyadenosine is a deoxyribonucleoside with the chemical formula C₁₀H₁₃N₅O₃. It is a derivative of adenosine. High presence of it can kill T lymphocytes and kill those cells, leading to adenosine deaminase severe combined immunodeficiency disease, also known as ADA-SCID.

Adenosine, Guanosine, Deoxyguanosine, Deoxyadenosine are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: guanosine, deoxyguanosine, adenosine, and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and H3PO4 as a buffer. UV detection at 210 nm.

Primesep 200 separates with baseline resolution the nucleosides (deoxyuridine, deoxyguanosine, deoxycytidine, deoxyadenozine) by a combination of cation exchange and reversed phase. These compounds are not retained on a traditional C18 column. Primesep 200 has an embedded anionic functional group which helps retain polar compounds by polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.