| CAS Number | 299-31-0 |
|---|---|
| Molecular Formula | C6H13O9P |
| Molecular Weight | 260.135 |
| InChI Key | NBSCHQHZLSJFNQ-GASJEMHNSA-N |
| LogP | -4.2 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Glucose 6-phosphate and Fructose 6-phosphate on Newcrom B Column
November 30, 2022
HPLC Method for Analysis of Glucose 6-phosphate and Fructose 6-phosphate on Newcrom B Column by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
Glucose 6-phosphate, also known as Robison ester, and Fructose 6-phosphate, known as Neuberg ester, are derivatives of glucose and fructose (respectively) that form during cellular metabolism. Glucose 6-phosphate is first formed by cells to ensure it does not diffuse out of the cell, and then through the process of glycolysis, it’s isomerized into Fructose 6-phosphate.
These 2 phosphorylated sugars can be retained, separated, and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of water, Acetonitrile (MeCN), and Formic acid (FA). This analytical method can be analyzed via any evaporative detector, including ELSD, CAD, and ESI-MS with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Glucose 6-phosphate and Fructose 6-phosphate
Condition
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 50% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 50C |
| Peak Retention Time | 11.4, 12.2 min |
Description
| Class of Compounds | Sugar |
| Analyzing Compounds | Glucose 6-phosphate and Fructose 6-phosphate |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsGlucose 6-Phosphate
HPLC Separation of Glucose 6- Phosphate and Glucose 1- Phosphate on Newcrom B Column
January 20, 2020
Separation type: Liquid Chromatography Mixed-mode
Structural isomer separation poses a challenging task when using reverse-phase chromatography. However both glucose
variants, which only differ to which carbon the phosphate group is attached to, can be separated in HPLC isocratically on a
reverse-phase mixed-mode anion-exchange Newcrom B column.
What differentiates, the Newcrom B phase from other silica-based
Analysis of glucose phosphate is important because glucose-6-phosphate is the product of the first step in glycolysis
metabolic pathway and glucose-1-phosphate is the first step in glycogenolysis. Glucose-6-phosphate is converted to
glucose-1-phosphate and vice versa by the phosphoglucomutase enzyme in biological systems.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glucose 6- Phosphate and Glucose 1- Phosphate
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 40 °C MS- compatible mobile phase |
| Class of Compounds | Hydrophilic, Sugar |
| Analyzing Compounds | Glucose 6- Phosphate, Glucose 1- Phosphate |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsGlucose 6-Phosphate
HPLC Separation of Sugar Phosphates
October 14, 2010
Sugar phosphates occur in biological systems. Sugar phosphates are parts of two metabolic pathways: glycolisis and pentose phosphate pathways. One of the purposes of sugar phosphates is to store and transfer energy in cells. Because of the presence of sugar fragment and phosphate moiety these molecules are very polar in nature. Mixed-mode chromatography was applied for separation of glucose and fructose phosphates and diphosphates. Compounds are separated based on anion-exchange properties with diphosphates retaining longer than mono phosphates. Monitoring is done by ELSD.
| Column | Primesep SB, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | AmFm – pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Sugars |
| Analyzing Compounds | Sodium, Glucose 6- phosphate, Fructose 6 –phosphate, Fructose 1.6 biphosphate, Ribulose 1.5 biphosphate |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsFructose 6-Phosphate
Glucose 6-Phosphate
Ribulose 1,5 Bisphosphate
Sodium
