| CAS Number | 15839-70-0 |
|---|---|
| Molecular Formula | C16H25N5O15P2 |
| Molecular Weight | 589.344 |
| InChI Key | LQEBEXMHBLQMDB-QIXZNPMTSA-N |
| LogP | -6.6 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Guanosine Diphosphate Fucose
January 30, 2026
If you are looking for optimized HPLC method to analyze Guanosine Diphosphate Fucose check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
Separation of Guanosine Diphosphate Mannose and Guanosine Diphosphate Fucose in Mixed-Mode Chromatography
December 5, 2013
Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analysis of Guanosine Diphosphate Mannose and Guanosine Diphosphate Fucose.
Two nucleotides containing different sugar fragments were separated on Primesep SB mixed-mode HPLC column using LC/MS compatible conditions. Method can be used for analysis and isolation of guanosine diphosphate mannose and guanosine diphosphate fucose. Mechanism of retention is weak reversed-phase and strong anion-exchange. retention is controlled by buffer concentration and buffer pH. Both compounds exhibit strong interaction providing good retention and separation.
| Column | Primesep SB, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | AmFm pH 2.9- 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Hydrophilic, Ionizable, Nucleotide sugar |
| Analyzing Compounds | Diphosphate Mannose, Guanosine Diphosphate Fucose |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsGuanosine Diphosphate Mannose

