| CAS Number | 620-61-1 |
|---|---|
| Molecular Formula | C17H25NO7S |
| Molecular Weight | 387.447 |
| InChI Key | VJFQPODMEGSXHC-BXSBZORQSA-N |
| Synonyms |
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Applications:
HPLC Method for Analysis of Hyoscyamine
May 10, 2016
Hyoscyamine is a natural plant belladonna alkaloid derivative and the levorotatory form of racemic atropine. It functions as a non-selective, competitive antagonist of muscarinic receptors and is used to treat a variety of stomach pains and intestinal problems as well as to decrease side effects of certain medications and insecticides. The drug does that by decreasing the acid production in the stomach and slowing down the natural movements of the gut. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Hyoscyamine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
| Column | Primesep 200, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 40% |
| Buffer | H3PO4 – 0.1%, |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Supplements |
| Analyzing Compounds | Hyoscyamine |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsHyoscyamine Sulfate
HPLC Separation of Active Compounds in Drug Formulation
January 13, 2005
An HPLC method for the separation of active drug compounds on a Primesep 200 column. The retention of compounds is achieved through reverse-phase, cation exchange and hydrophobic interactions. Benzoic acid, hyoscyamine sulfate and phenyl salicylate are baseline separated using simple mobile phases of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with a UV detector.
| Column | Primesep 200, 3.0×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | TFA |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 220 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Benzoic acid, Hyoscyamine Sulfate, Phenylsalicylate) |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsHyoscyamine Sulfate
Phenylsalicylate
