Lactic Acid Oligomers

Lactic Acid Oligomers

Synonyms

    Applications:

    HPLC Determination of Lactic Acid on Newcrom BH Column

    October 7, 2020


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    Lactic acid (milk acid) is a natural organic acid that plays an important role in biochemical processes.  It has a carboxylic and hydroxy- groups which, upon standing, can react with each other and form dimers, trimers and higher oligomers.  Lactic acid is a polar molecule that does not retain on reversed-phase columns. Newcrom BH mixed-mode anion-exchange column is used for the separation of lactic acid and its oligomers by a combination of weak reversed-phase and anion-exchange mechanisms. Lactates can be monitored by low UV, ELSD, CAD  or LC/MS.

    Condition

    Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer HClO4 – 0.1%
    Flow Rate 1 ml/min
    Detection UV 210 nm

     

    Description

    Class of Compounds
    Acid, Hydrophilic, Ionizable
    Analyzing Compounds Lactic Acid

     

    Application Column

    Newcrom BH

    The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

    Select options
    Application Analytes:
    Lactic Acid
    Lactic Acid Oligomers
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Analysis of Lactic Acid on Primesep D Column

    December 5, 2013

     

    Lactic acid (milk acid) is a hydrophilic acid which plays important role in many bioprocesses. Lactic acid has a tendency to self-condense to form dimers, trimers and high molecular weight oligomers. Lactic acid and it’s oligomers are retained by a combination of weak reversed-phase and weak anion-exchange mechanisms on Primesep D mixed-mode HPLC column. Retention time is controlled by amount of acetonitrile, buffer concentration and buffer pH. Method is compatible with LC/MS and other detection techniques and can be used for analysis of lactic acid in various matrices.

    Condition

    Column Primesep D, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 30/70%
    Buffer AmAc pH 4.0- 20 mM
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Acid, Hydrophilic, Ionizable
    Analyzing Compounds Lactic Acid

     

    Application Column

    Primesep D

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Lactic Acid
    Lactic Acid Oligomers

    Application Detection:
    ELSD Detection
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of Lactic Acid and Its Oligomers

    July 15, 2010


    Lactic acid (milk acid) is natural organic acid that plays an important role in biochemical processes. It has carboxylic and hydroxy-groups which upon standing can react with each other and form dimers, trimers and higher oligomers. Lactic acid is a polar molecule that produces no retention on reversed-phase column. Primesep B2 mixed-mode anion-exchange column is used for the separation of lactic acid and its oligomers by a combination of weak reversed-phase and anion-exchange mechanisms. Oligomers of lactic acid can be converted to lactic acid by hydrolysis. Primesep B2 column can be used for the analysis of lactic acid alone and lactic acid in the mixture of oligomers. Lactates can be monitored by low UV, ELSD or LC/MS. In case of ELSD, CAD or LC/MS, phosphoric acid in the mobile phase needs to be replaced with buffer compatible with these detection techniques (ammonium formate or TFA).

    Condition

    Column Primesep B2, 3.2×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 30/70%
    Buffer H3PO4 – 0.1%
    Flow Rate 0.5 ml/min
    Detection UV 210 nm

     

    Description

    Class of Compounds
    Acid, Hydrophilic, Ionizable
    Analyzing Compounds Lactic Acid

     

    Application Column

    Primesep B2

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Lactic Acid
    Lactic Acid Oligomers
    Organic Acids

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.