Lecithin

Lecithin

Synonyms

    Applications:

    Uv-Vis Spectrum of Lecithin

    March 13, 2026

    Access the UV-Vis Spectrum SIELC Library

    If you are looking for optimized HPLC method to analyze Lecithin check our HPLC Applications library

    For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
    For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

    Application Analytes:
    Lecithin
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of Lecithin and Soy lecithin on Primesep N Column

    July 2, 2013

    Lecithin is a naturally occurring compound composed of phosphoric acid, choline, fatty acids, glycerol, glycolipids, triglycerides and phospholipids.

    Condition
    Column Primesep N, 4.6×150 mm, 5 µm, 100A
    Mobile Phase Gradient MeCN 92-75%, 20 min
    Buffer Gradient AmFm pH 3.0- 8-20 mM, 20 min
    Flow Rate 1.0 ml/min
    Detection ELSD, 40C

     

    Description
    Class of Compounds
    		 Aminoacid, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Lecithin

     

    Application Column

    Primesep N

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Lecithin
    Soy Lecithin
    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.