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Separation of Mandelic Acid Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds  Primesep 100 separates a mixture of amino acids (tyrosine, phenylalanine), organic acids (benzoic acid, mandelic acid), amines (benzylamine, pyridine), and neutrals (benzonitrile, toluene) in one HPLC run by combining reversed-phase, cation-exchange, and polar interactions. The method is tunable and peak order can be changed significantly by adjusting acetonitrile and trifluoroacetic acid concentrations. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and compatible with UV, mass spec (LC/MS) and evaporative light scattering (ELSD) detection. Application Analytes: Amino Acids Benzoic Acid Benzonitrile Benzylamine Mandelic Acid Phenylalanine Pyridine Toluene Tyrosine Application Detection: UV Detection HILIC Separation of Carboxylic Acids  Hydrophilic acids are separated on Obelisc N mixed-mode HILIC column. Seven carboxylic acids are separated based on their polarity and pKa values. Changes in ionization states of acids and stationary phase can be used to control elution order of organic and inorganic acids. Application Analytes: Hydroxybenzoic Acid Succinic Acid Mandelic Acid Methylmalonic Acid Malic Acid Fumaric Acid Tartaric Acid Application Detection: ELSD/MS Detection |
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