Metformin

Metformin structural formula

CAS Number657-24-9
Molecular FormulaC4H11N5
Molecular Weight129.168
InChI KeyXZWYZXLIPXDOLR-UHFFFAOYSA-N
LogP-1.37
Synonyms
  • Metformin
  • N,N-Dimethyltriimidodicarbonic diamide
  • Imidodicarbonimidic diamide, N,N-dimethyl-
  • 657-24-9
  • Imidodicarbonimidic diamide, N,N-dimethyl-
  • 1,1-Dimethylbiguanide
  • Biguanide, 1,1-dimethyl-
  • Cidophage
  • Dimethylbiguanide
  • Emiphage
  • Ficonax
  • Fluamine
  • Flumamine
  • Gliguanid
  • Haurymelin
  • metformina
  • metformine
  • Metiguanide
  • Metphage
  • N,N-Dimethylbiguanide
  • N,N-Dimethyldiguanide
  • N,N-Dimethylimidodicarbonimidic diamide
  • N,N-Dimethyl-imidodicarbonimidic diamide
  • N1,N1-Dimethylbiguanide
  • N'-Dimethylguanylguanidine
  • Diabetosan
  • Dimethyldiguanide
  • EINECS 211-517-8
  • Glifage
  • Islotin
  • Metforminum
  • UNII-9100L32L2N
  • 1,1-Dimethyl biguanide
  • 1-carbamimidamido-N,N-dimethylmethanimidamide
  • DMBG
  • Diabefagos
  • Diabex
  • Diaformin
  • Dianben
  • Fortamet
  • Glucophage
  • Glumetza
  • Meguan
  • Metformin
  • Obimet
  • Riomet

Applications:

HPLC Method for Analysis of Metformin on Primesep 100 Column

February 1, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Metformin on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Moroxydine on Primesep 100 Column
HPLC Method for Analysis of Metformin on Primesep 100 Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Metformin

Biguanides, including Moroxydine, Metformin, Proguanil, and Phenformin, are a group of organic compounds that share a common structural feature, namely the presence of two guanidine groups.

Metformin is an oral antidiabetic medication used to treat type 2 diabetes. It works by reducing glucose production in the liver and improving insulin sensitivity in peripheral tissues. Metformin is one of the most widely prescribed drugs for diabetes management.

These biguanides exhibit various pharmacological activities, and while some are primarily used for antidiabetic purposes, others have applications in antiviral or antimalarial treatments. It’s important to note that the safety and efficacy of these drugs depend on proper medical supervision and adherence to prescribed dosages.

Metformin can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 240 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O 75/25%
BufferAmFm pH 3.0  – 30 mM
Flow Rate1.0 ml/min
DetectionUV 240 nm
Samples 0.5 mg/ml
Injection volume2 µl
LOD*8 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Biguanides
Analyzing CompoundsMetformin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Metformin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation Biguanides such as Moroxydine, Metformin, Proguanil and Phenformin on Primesep 100 Column

February 1, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Moroxydine, Metformin, Proguanil and Phenformin on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC method for separating Biguanides such as Moroxydine , Metformin, Proguanil and Phenformin on Primesep 100 Column
HPLC Method for Analysis of Metformin, Proguanil hydrochloride, Phenformin, Moroxydine on Primesep 100 Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Moroxydine , Metformin, Proguanil and Phenformin

Biguanides, including Moroxydine, Metformin, Proguanil, and Phenformin, are a group of organic compounds that share a common structural feature, namely the presence of two guanidine groups.

Moroxydine:

  • Moroxydine is an antiviral medication used in the treatment of influenza. It is believed to inhibit the early stages of viral replication.

Metformin:

  • Metformin is an oral antidiabetic medication used to treat type 2 diabetes. It works by reducing glucose production in the liver and improving insulin sensitivity in peripheral tissues. Metformin is one of the most widely prescribed drugs for diabetes management.

Proguanil:

  • Proguanil is an antimalarial medication often used in combination with other drugs, such as atovaquone. It is used for both prevention and treatment of malaria. Proguanil is a prodrug, and its active form inhibits the development of the malaria parasite in the liver.

Phenformin:

  • Phenformin is an antidiabetic biguanide that was previously used to treat type 2 diabetes. However, due to the risk of lactic acidosis (a serious side effect), it has been largely withdrawn from the market in many countries.

These biguanides exhibit various pharmacological activities, and while some are primarily used for antidiabetic purposes, others have applications in antiviral or antimalarial treatments. It’s important to note that the safety and efficacy of these drugs depend on proper medical supervision and adherence to prescribed dosages.

Biguanides such as Moroxydine , Metformin, Proguanil and Phenformin be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 240 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O 70/30%
BufferAmFm pH 3.0  – 30 mM
Flow Rate1.0 ml/min
DetectionUV 240 nm
Samples1. 0.1 mg/ml
2. 0.1 mg/ml
3. 0.1 mg/ml
4. 0.1 mg/ml
Injection volume10 µl
LOD*1. 7 ppb
2. 7 ppb
3. 17 ppb
4. 18 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Biguanides
Analyzing CompoundsMetformin, Proguanil hydrochloride, Phenformin, Moroxydine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Metformin
Moroxydine
Phenformin
Proguanil hydrochloride

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Guanylurea and Metformin on Primesep 100 Column

November 27, 2023

HPLC Method for Analysis of Guanylurea, Metformin on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Guanylurea, Metformin on Primesep 100 Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Guanylurea, Metformin

Guanylurea is a known degradation product of metformin. When metformin undergoes biotransformation in the body, one of the metabolic pathways leads to the formation of guanylurea. This process is a part of the pharmacokinetics of metformin, where the drug is metabolized and eventually excreted from the body.

The conversion of metformin to guanylurea is significant for a few reasons:

Pharmacokinetics: Understanding the metabolism of metformin, including its conversion to guanylurea, is important for comprehending its action in the body, its elimination, and any potential metabolite-related effects.

Environmental Impact: The presence of pharmaceuticals and their metabolites, like guanylurea from metformin, in the environment (especially in water sources) has become a topic of environmental concern. This is because these compounds can have effects on aquatic life and potentially re-enter the human water supply.

Safety and Efficacy: Knowing the metabolites of a drug helps in assessing its safety and efficacy. While guanylurea is not known to have significant pharmacological effects in the context of metformin’s use as a diabetes medication, its identification is essential for a complete understanding of the drug’s profile.

Guanylurea: This is the main known metabolite of metformin. It is formed through the breakdown of metformin in the liver. Despite being the primary metabolite, guanylurea is considered to have minimal pharmacological activity, especially in the context of the glucose-lowering effects of metformin.

Guanylurea and Metformin can be retained, separated and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid as a buffer. This analysis method can be detected using UV at 200 nm.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 30/70%
BufferGradient H2SO4 -0.1-0.5%, 10 min
Flow Rate1.0 ml/min
DetectionUV 200 nm
SampleGuanylurea phosphate 0.2 mg/ml in H2O and 1,1-Dimethylbiguanide hydrochloride 0.2 mg/ml in H2O

Class of Compounds
Ureas
Analyzing CompoundsGuanylurea, Metformin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Guanylurea
Metformin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of Metformin

July 8, 2011

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Metformin is a biguanide-based anti-diabetic drug. It is a very polar organic compound that has no retention on reversed-phase column unless an ion-pairing reagent is employed. Metformin was retained on a Primesep 200 column based on cation-exchange mechanism. Method can be used for analysis of metformin and related substances in pharmaceutical formulations. Due to low UV activity a more UV-transparent mobile phase is required. Metformin can be also monitored by ELSD and LC/MS with corresponding mobile phase (TFA, ammonium formate or ammonium acetate).

Condition

Column Primesep 200, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 0.5 ml/min
Detection UV, 230 nm

 

Description

Class of Compounds
Drug,  Anti-diabetic medication, Hydrophilic, Ionizable
Analyzing Compounds Metformin

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Metformin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.