Compounds  →  Methylene Blue

Separation of Methylene Blue

HPLC Separation of Dyes


Various dyes are widely used in food, textile and pharmaceutical industries. Dies are aromatic compounds containing ionizable groups. Methylene blue, crystal violet lactone and crystal violet were separated on a Primesep D HPLC column. Method can be used for separation of other dyes and pigments that are basic and hydrophobic in nature.



Application Analytes:

Crystal Violet
Crystal Violet Lactone
Methylene Blue

Application Detection:

UV Detection

HPLC Dye Separation on SHARC1 Column



Application Analytes:

Methyl Orange
Methyl Red
Methylene Blue
Nile Blue A

Application Detection:

UV Detection

Analysis of Methylene Blue Purity in Reversed-Phase Cation-Exchange and Reversed-Phase Cation-Exclusion Modes

Methylene blue is a heterocyclic dye with a wide range of use in biology and chemistry. Purity of dyes rarely exceed 70% and separation of methylene blue and related impurities is required. Two alternative methods for purity analysis were developed on Primesep C and Primesep D mixed-mode HPLC columns. Primesep C is a reversed-phase cation-exchange (anion-exclusion column) column and Primesep D is reversed-phase anion-exchange (cation-exclusion) column. Various buffers can be used for the method. Depending on the buffer, method can be compatible with UV, LC/MS and prep chromatography. Method can be used for prep separation of methylene blue and related impurities.



Application Analytes:

Methylene Blue

Application Detection:

UV Detection
ELSD/MS Detection

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