CAS Number | 200-441-0 |
---|---|
Molecular Formula | C6H5NO2 |
Molecular Weight | 123.111 |
InChI Key | PVNIIMVLHYAWGP-UHFFFAOYSA-N |
LogP | 0.4 |
Synonyms |
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Applications:
Analysis of Nicotinic Acid and Nicotinamide (Vitamin B3) on Newcrom AH Column
July 6, 2015
Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Newcrom AH was used to separate nicotinamide and nicotinic acid. Newcrom AH separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.
Column | Newcrom AH, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 50/50% |
Buffer | H3PO4 – 0.5% |
Flow Rate | 1.0 ml/min |
Detection | UV, 210 nm |
Class of Compounds |
Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds | Nicotinic Acid, Nicotinamide, Vitamin B3 |
Application Column
Newcrom AH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsNicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
HPLC Separation of Niacin on L1 Column
July 2, 2013
Column | Legacy L1, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeOH/MeCN |
Buffer | Hexanesulfonate/AcOH |
Flow Rate | 1.0 ml/min |
Detection | UV, 250 nm |
Class of Compounds |
Drug, Cholesterol medication, Vitamin, Hydrophobic, Ionizable |
Analyzing Compounds | Niacin |
Application Column
Legacy L1
SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.
Select options