Niacin

Niacin

CAS Number200-441-0
Molecular FormulaC6H5NO2
Molecular Weight123.111
InChI KeyPVNIIMVLHYAWGP-UHFFFAOYSA-N
LogP0.4
Synonyms
  • nicotinic acid
  • niacin
  • Pyridine-3-carboxylic acid
  • 3-pyridinecarboxylic acid
  • 3-Carboxypyridine
  • wampocap
  • vitamin B3
  • Acidum nicotinicum
  • Apelagrin
  • Pellagrin
  • Akotin
  • Daskil
  • Efacin
  • Pelonin
  • Linic
  • nicamin
  • nicobid
  • nicocap
  • nicolar
  • Enduracin
  • Nicodelmine
  • Niconacid
  • Nicotinipca
  • Pellagramin
  • Direktan
  • Niaspan
  • Nicacid
  • Nicangin
  • Peviton
  • Bionic
  • Diacin
  • Nicyl
  • Nyclin
  • Niac
  • Vitaplex N
  • Davitamon PP
  • Nico-Span
  • Tega-Span
  • Nicocidin
  • Nicocrisina
  • Niconazid
  • Nicoside
  • Nicotamin
  • Nicotene
  • Nicovasan
  • Nicovasen
  • Nipellen
  • SK-Niacin
  • Naotin
  • Nicodan
  • Nicodon
  • Niconat
  • Nicosan 3
  • Nicosyl
  • Nicotil
  • Tinic
  • 3-Carboxylpyridine
  • Nicotine acid
  • Niacor

Applications:

Analysis of Nicotinic Acid and Nicotinamide (Vitamin B3) on Newcrom AH Column

July 6, 2015


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Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Newcrom AH  was used to separate nicotinamide and nicotinic acid.  Newcrom AH separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.

Condition

Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
 Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Nicotinic Acid, Nicotinamide, Vitamin B3

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Niacin
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Niacin on L1 Column

July 2, 2013

 

Condition

Column Legacy L1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeOH/MeCN
Buffer Hexanesulfonate/AcOH
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Cholesterol medication, Vitamin, Hydrophobic, Ionizable
Analyzing Compounds Niacin

 

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Select options
Application Analytes:
Niacin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.