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Separation of Norepinephrine Separation of Epinephrine Norepinephrine  Primesep 200 separates the catecholamines, norepinephrine and epinephrine, less than 8 minutes. Norepinephrine and epinephrine are baseline resolved by a combination of reversed-phase, and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Epinephrine Norepinephrine Application Detection: UV Detection HPLC Separation of Polar Compounds
Application Analytes: Aspartic Acid Dopamine Glutamic Acid Norepinephrine 5-Hydroxytryptophan gamma-Aminobutyric Acid (GABA) Application Detection: ELSD/MS Detection Separation of Serotonin, Dopamine, and Related Compounds  Catecholamines are chemical compounds derived from the amino acid tyrosine containing catechol and amine groups. Some of them are biogenic amines. Retention of compounds of catecholamine pathway is achieved on Obelisc N column. All polar compounds are well retained by combination of HILIC and ion-exchange mechanisms. Obelisc N columns produce very good peak shapes for all analytes. Method is very sensitive to amount of ACN, buffer and buffer pH. Retention time changes with variation of main parameters. This method can be used for quantitation of biogenic amines and related compounds (homovanillic acid, dihydroxyphenyl acetic acid, serotonin, dopamine, epinephrine, hydroxytryptophan, epinephrine and DOPA) in urine, blood and other biological fluids. Further optimization of this HPLC method can be used during screening and validation. Amines and acids can be analyzed in the same run and retained by combination of polar organic mode, cation-exchange and anion-exchange modes. Various buffers within specified pH can be employed (ammonium formate, ammonium acetate, sodium phosphate, etc.). Application Analytes: Homovanillic Acid DOPAC (Dihydroxyphenylacetic Acid) Serotonin Dopamine Epinephrine Hydroxytryptophan Norepinephrine DOPA (3,4-dihydroxy-L-phenylalanine) Application Detection: UV Detection HPLC Separation of Neurotransmitters and Related Drugs  Epinephrine and norepinephrine (adrenaline and noradrenaline) are hormones and neurotransmitters. Epinephrine is synthesized from norepinephrine in a synthetic pathway shared by all catecholamines, including L-dopa, dopamine, norepinephrine, and epinephrine. Phenylephrine is used as a decongestant, available as an oral medicine or as a nasal spray. Phenylephrine is now the most common over-the-counter (OTC) decongestant. All three compounds are used in various drug compositions. Separation of epinephrine and norepinephrine is a challenging task due to polarity and close properties of two compounds. Epinephrine, norepinephrine and phenylephrine are separated in this method on Obelisc R mixed-mode HPLC columns. Method is very sensitive to variation of pH and pH adjustment can be used to achieve desired selectivity and retention time. Other catecholamines can be analyzed using this HPLC method. Method can be used as stability indicating or impurity profiling approach to analysis of neurotransmitters in drug formulation, blood, serum and urine. Application Analytes: Epinephrine Norepinephrine Phenylephrine Application Detection: UV Detection Separation of Catecholamines on Primesep 100 Column with Phosphate Buffers
The catecholamine neurotransmitters are amino-acid derivatives of tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine and baseline are resolved on a Primesep 100 column with UV-transparent phosphate buffer. This method can be used for analysis of catecholamines and related impurities in various matrices. Peak order and retention time can be changed by changing the amount of ACN, buffer concentration and buffer pH. Various buffers can be used to accommodate desired detection technique. Primesep 100 is a reversed-phase cation-exchange mixed-mode column that can be used for analysis of polar neutral, polar ionizable, polar zwitter-ionic, hydrophobic neutral, and hydrophobic ionic compounds in the same run. Column can be operated in reverse-phase, cation-exchange, anion-exclusion, HILIC and mixed-modes depending on the mobile phase selection and nature of analytes. Column is compatible with LC/MS and does not require use of ion-pairing reagents. Application Analytes: DOPA (3,4-dihydroxy-L-phenylalanine) Dopamine Epinephrine Norepinephrine Phenylalanine Tyrosine Application Detection: UV Detection |
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