HPLC Method for PMIDA (Phosphonomethyliminodiacetic acid) on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of PMIDA (Phosphonomethyliminodiacetic acid).

N-(Phosphonomethyl)iminodiacetic acid, also known as PMIDA, is a key intermediate in the making of glyphosate. It has the chemical formula (HO)₂P(O)CH₂N(CH₂CO₂H)₂. You can find detailed UV spectra of PMIDA and information about its various lambda maxima by visiting the following link.

PMIDA (Phosphonomethyliminodiacetic acid) can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.

HPLC Method for PMIDA (Phosphonomethyliminodiacetic acid), Glyphosate on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of PMIDA (Phosphonomethyliminodiacetic acid), Glyphosate.

Glyphosate is a broad-spectrum herbicide. It works through inhibiting the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase. The enzyme is essential for producing amino acids within the plant.  Glyphosate is used across agriculture and forestry, as well as rare aquatic environments.  It’s chemical formula is C₃H₈NO₅P. You can find detailed UV spectra of Glyphosate and information about its various lambda maxima by visiting the following link.

N-(Phosphonomethyl)iminodiacetic acid, also known as PMIDA, is a key intermediate in the making of glyphosate. It has the chemical formula (HO)₂P(O)CH₂N(CH₂CO₂H)₂. You can find detailed UV spectra of PMIDA and information about its various lambda maxima by visiting the following link.

PMIDA (Phosphonomethyliminodiacetic acid), Glyphosate can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.

If you are looking for optimized HPLC method to analyze PMIDA (Phosphonomethyliminodiacetic acid) check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Glyphosate and intermediates/impurities of production are separated on an Obelisc N HILIC/ion-exchange column by a combination of HILIC and ion-exchange mechanism. Method can be used in analysis of glyphosate, iminodiacetic acid, (N-phosphonomethyl)-imminodiacetic acid, diethanolamine and related impurities in reaction mixtures, waste and ground waters. Detection techniques are LC/MS, ELSD, CAD and UV.

Glyphosate is a broad spectrum herbicide used to kill weeds. It is the most used herbicide. Glyphosate is an aminophosphonic analogue of the natural amino acid glycine. Glyphosate and its intermediates are very polar ionic compounds derived from glycine. Neither of intermediates can be retained on traditional reversed-phase columns. Two methods for glyphosate intermediates were developed on Primesep B, Primesep B2 and Primesep 100 columns.