Access the UV-Vis Spectrum SIELC Library

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For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Fatty acids are carboxylic acids with a long unbranched carbon chain. They are hydrophobic compounds with different number of carbons. Most naturally occurring fatty acids have a chain of an even number of carbon atoms. Three fatty acids are separated on a Primesep 100 HPLC column by reversed-phase mechanisms. This general HPLC method and mixed-mode column can be used for separation of other fatty acids.