Pantothenic Acid

Panthothenic Acid

CAS Number79-83-4
Molecular FormulaC9H17NO5
Molecular Weight219.237
InChI KeyGHOKWGTUZJEAQD-ZETCQYMHSA-N
LogP-1.1
Synonyms
  • Vitamin B5
  • pantothenic acid
  • D-pantothenic acid
  • 79-83-4
  • pantothenate
  • Chick antidermatitis factor
  • (+)-Pantothenic acid
  • (R)-pantothenate
  • PANTOTHENOIC ACID
  • Kyselina pantothenova [Czech]
  • calcium pantothenate
  • HSDB 1020
  • Pantothenic Acid [BAN]
  • D(+)-N-(2,4-Dihydroxy-3,3-dimethylbutyryl)-beta-alanine
  • (D)-(+)-Pantothenic acid
  • BRN 1727064
  • (R)-pantothenic acid
  • UNII-19F5HK2737
  • Zinc pantothenate
  • beta-Alanine, N-(2,4-dihydroxy-3,3-dimethyl-1-oxobutyl)-, (R)-
  • D-(+)-pantothenic acid
  • EINECS 201-229-0
  • (D,+)-N(alpha-gamma-Dihydroxy-beta,beta-dimethylbutyryl)-beta-alanine
  • N-(2,4-Dihydroxy-3,3-dimethylbutyryl)-beta-alanine
  • CHEBI:46905
  • N-[(2R)-2,4-DIHYDROXY-3,3-DIMETHYLBUTANOYL]-BETA-ALANINE
  • 19F5HK2737
  • (R)-3-(2,4-Dihydroxy-3,3-dimethylbutanamido)propanoic acid
  • AK116516
  • (R)-N-(2,4-Dihydroxy-3,3-dimethyl-1-oxobutyl)-beta-alanine
  • Pantothenic acid (BAN)
  • Pantothenic acid, D-
  • (+)-Pantothenate
  • 3-[[(2R)-2,4-dihydroxy-3,3-dimethylbutanoyl]amino]propanoic acid
  • Pantothenate, Zinc
  • PAU
  • B5, Vitamin
  • Vitamin B 5
  • Kyselina pantothenova
  • B 5, Vitamin
  • Pantothenate, Calcium
  • PANTOTHENOate
  • d-(+)-pantothenate
  • Pantothen Pharmaselect
  • pantothenate;vitamin B5

Applications:

HPLC Method for Separation of 10 Water-Soluble Vitamins on Chromni Column

September 17, 2025

HPLC Method for Separation of 10 Water-Soluble Vitamins on Chromni™ by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for separation of Vitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin

Riboflavin (B2), Nicotinic Acid (B3), Nicotinamide (B3), Pyridoxine (B6), Folic Acid (B9), Cyanocobalamin (B12), Thiamine Pyrophosphate (coenzyme of B1), Thiamine (B1), Pantothenic Acid (B5), Biotin (B7) are water soluble vitamins with a key function of energy metabolism. These coenzymes are responsible for converting food into usable energy.

Vitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN). Detection is performed using UV.

ColumnChromni™, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseGradient of MeCN
BufferGradient of Phosphoric Acid
Flow Rate1.0 ml/min
DetectionUV 275 nm, 200 nm

Class of CompoundsVitamins
Analyzing CompoundsVitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin

Application Column

Chromni™

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Biotin
Cyanocobalamin
Folic Acid
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pantothenic Acid
Thiamine diphosphate (Thiamine pyrophosphate)
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of Active Drug in a Formulation

October 4, 2010


HPLC method for separation of active ingredients of drug/supplemental composition was developed on an Obelisc R trimodal HPLC column. Compounds are retained by combination of reversed-phase, cation-exchange and anion-exchange mechanisms. Compounds are well separated, and method can be used for quantitation of pyridoxine, ascorbic acid, niacinamide, pantothenic acid, caffeine and riboflavin in a mixture or as separate compounds in various complex mixtures. Various detection techniques can be applied for quantitation (ELSD, UV, LC/MS, Corona). This HPLC method can be adopted as general approach for analysis of active drug components in various formulations.

Condition
Column Obelisc R , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O  -5/95%
Buffer NaHPO4 pH 3.0 – 30 mM
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description
Class of Compounds
 Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine, Ascorbic acid, Niacinamide, Pantothenic acid, Caffeine, Riboflavin

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:

Ascorbic Acid
Caffeine
Niacinamide
Pantothenic Acid
Vitamin B6 (Pyridoxine)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.