Polyethylene Glycol

Synonyms

    Applications:

    HPLC Method For Analysis of Ethylene Glycol and Polyethylene Glycol (PEG) on Primesep AP Column

    September 14, 2021

    HPLC Method for Ethylene Glycol, Polyethylene Glycol on Primesep AP by SIELC Technologies


    High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylene glycol, PEG 200, PEG 400.

    Polyethylene glycol (PEG) has a low toxicity and is used in a variety of products (creams, laxatives, lubricants). When attached to various protein medications, polyethylene glycol allows a slowed clearance of the carried protein from the blood. This makes for a longer acting medicinal effect, reduces toxicity, and allows longer dosing intervals.

    HILIC separation can be used to determine polyethylene glycol content in various formulations using ELSD, RI detection, or LC/MS detection. HPLC separation of oligomers can be achieved by changing the amount of acetonitrile.

    ColumnPrimesep AP, 4.6 x 150 mm, 5 µm, 100 A, dual ended
    Mobile PhaseMeCN/H2O – 95/5%
    BufferNo
    Flow Rate1.0 ml/min
    DetectionUV, 200 nm, RI
    LOD*Ethylene glycol 6 ppm (UV)
    *LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
    Class of Compounds
    		Diol
    Analyzing CompoundsEthylene Glycol, Polyethylene Glycol

    Application Column

    Primesep AP

    Column Diameter: 4.6 mm
    Column Length: 150 mm
    Particle Size: 5 µm
    Pore Size: 100 A
    Column options: dual ended

    Add to cart
    Application Analytes:
    Ethylene Glycol
    Polyethylene Glycol
    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HILIC HPLC Separation of Polyethylene Glycol (PEG)

    August 22, 2008

    Polyethylene glycol (PEG)  has low toxicity and is used in a variety of products (creams, laxatives, lubricants). When attached to various protein medications, polyethylene glycol allows a slowed clearance of the carried protein from the blood. This makes for a longer acting medicinal effect, reduces toxicity, and allows longer dosing intervals. HILIC separation of polyethylene glycol can be used to determine polyethylene glycol content in various formulations using ELSD, RI detection, or LC/MS detection. HPLC separation of oligomers can be achieved by changing amount of acetonitrile or buffer pH.

    Application Column

    Obelisc N

    SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

    Select options
    Application Analytes:
    Polyethylene Glycol
    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.