Compounds  →  Polysorbate 20

Separation of Polysorbate 20

HPLC Retention of Polysorbate on Primesep D Column



Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers. All oligomers are separated on Primesep D HPLC column. One of the oligomers can be used in quantitating polysorbate in various drug and food formulations.

Application Analytes:

Polysorbate
Polysorbate 20
Polysorbate 80

Application Detection:

ELSD/MS Detection

HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar



Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers.

Application Analytes:

Amino Acids
Polysorbate
Polysorbate 20
Polysorbate 80
Tween 20
Tween 80
Histidine
Glycine
Sucrose

Application Detection:

ELSD/MS Detection

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