Compounds  →  Saccharin

Separation of Saccharin

HPLC Separation of Saccharin and Sorbitol


Sorbitol and saccharin are two sugar substitutes used in food and drinks. Separation of sorbitol and saccharin was achieved on a Primesep N HILIC column using acetonitrile/water with ammonium formate. Compounds were monitored by combination of UV and ELSD. Method is compatible with LC/MS detection.


Application Analytes:

Saccharin

Application Detection:

UV Detection
Separation of Alginic Acid and Related Products



Alginate is used in various pharmaceutical preparations. Chemically, it is a linear copolymer with homopolymeric blocks of (1-4)-linked ?-D-mannuronate (M) and its C-5 epimer ?-L-guluronate (G) residues, respectively, covalently linked together in different sequences or blocks. Alginic acid can be separated from benzoate, citric acid and saccharin by mixed-mode chromatography on Primesep C HPLC column. This method can be used to quantitate alginic acid, citric acid or saccharin in complex mixtures. Various detection technique can be used (UV, ELSD, LC/MS), based on mobile phase selection.
Application Analytes:

Alginic Acid
Citric Acid
Benzoic Acid
Saccharin

Application Detection:

UV Detection
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