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Separation of Sodium Reverse Elution Order in Separation of Alkali Cation  Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD). Application Analytes: Lithium Potassium Sodium Application Detection: ELSD/MS Detection Separation of Guanidine  Primesep 100 separates guanidine from its sodium counter ion on a short 50 mm column by cation exchange. Guanidine is found in urine as a product of protein metabolism and is also used in the manufacture of plastics and explosives. Guanidine is detected by UV detection at 200 nm, but UV cannot detect sodium ion. If an evaporative light scattering detector (ELSD) is used, both components can be detected. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA). Application Analytes: Guanidine Sodium Application Detection: UV Detection ELSD/MS Detection Complex of Cations on Primesep C is Effected by Organic Modifier  Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. In addition, the elution order can be rearranged by simply changing the organic modifier in the mobile phase from acetonitrile to methanol. The mobile phase mixture of water, acetonitrile (MeCN, ACN) or methanol (MeOH) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD). Application Analytes: Lithium Potassium Sodium Application Detection: ELSD/MS Detection Separation of Metal Ions on Primesep 100 Column  Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible. Application Analytes: Calcium Chloride Lithium Magnesium Potassium Sodium Zinc Application Detection: ELSD/MS Detection Zn and Na Quantitation in Drug Substances  Primesep 100 allows the quantitation of sodium and zinc cations in a drug product sample. The cations are retained by cation exchange and the drug product is retained by hydrophobic, reversed-phase mechanisms. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD) and UV detection. Application Analytes: Chloride Sodium Zinc Application Detection: UV Detection ELSD/MS Detection Simultaneous Separation of Cations and Anions  Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium captions by cation exchange. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD). Application Analytes: Bromide Chloride Potassium Sodium Application Detection: ELSD/MS Detection Effect of Concentration of Organic Modifier on Retention of Cation and Anions  Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium cations by cation exchange. The retention can be adjusted by changing the water/acetonitrile ratio in the mobile phase. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate with evaporative light scattering detection (ELSD). Application Analytes: Bromide Chloride Potassium Sodium Application Detection: ELSD/MS Detection Separation of Sodium, Potassium, Lithium, and Ammonium in Mixed-Mode HPLC
Application Analytes: Ammonium Ion Lithium Potassium Sodium Application Detection: ELSD/MS Detection HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC  Sulfate and phosphate are separated on Primesep B2 and Primesep D column by anion-exchange mechanism. Because both sulfate and phosphate ions are not UV-active, ELSD is used to monitor separation of both anions. Method can be used to quantitate phosphate and sulfate in various pharmaceutical and chemical formulation, products and solution, drinking and ground water. Retention time is adjusted by increase or decrease of buffer concentrations. Two anions can be retained and separated on a very short column due to strong ion-exchange interaction. Method shows good reproducibility and versatility. Application Analytes: Sodium Sulfate Phosphate Application Detection: ELSD/MS Detection Separation of Sodium, Tetramethylammonium and Tetrabutylammonium on Primesep 200 Column
Hydrophilic and hydrophobic quaternary amines, along with sodium ion, were separated by mixed-mode chromatography on a Primesep 200 column. Mechanism of retention for sodium and tetramethylammonium ions is cation exchange, while the tetrabutylammonium ion is retained by combination of reversed-phase and cation-exchange mechanisms. All three compounds are not UV-active and monitoring is done by ELSD/CAD. Application Analytes: Sodium Tetrabutylammonium Tetramethylammonium Application Detection: ELSD/MS Detection HPLC Separation of Citrulline and Arginine  Application Analytes: Arginine Sodium Citrulline Application Detection: ELSD/MS Detection HPLC Separation of Alkyl Sulfonic Acids
Application Analytes: Heptanesulfonic Acid Hexanesulfonic Acid Octanesulfonic Acid Sodium Application Detection: ELSD/MS Detection HPLC Separation of Surfactants  Application Analytes: Sodium Cetylpyridinium Chloride Benzalkonium Chloride Triton X100 Application Detection: UV Detection HPLC Separation of Sodium, Chloride, Bromide, and Iodine
Application Analytes: Bromide Chloride Iodine Sodium Application Detection: ELSD/MS Detection HPLC Separation of Sugar Phosphates
Application Analytes: Fructose 1,6 Bisphosphate Fructose 6-Phosphate Glucose 6-Phosphate Ribulose 1,5 Bisphosphate Sodium Application Detection: ELSD/MS Detection Simultaneous Determination of Sodium and Sulfate Ions on Obelisc N Mixed-mode HILIC Column  Inorganic cations and anions are usually analyzed by two different methods - cation-exchange and anion-exchange. This requires development and validation of two methods. Both ions can be analyzed on mixed-mode HILIC column with ELSD detection. Both ions are retained by combination of HILIC and ion-exchange mechanism. Application Analytes: Sodium Sulfate Application Detection: ELSD/MS Detection Simultaneous Analysis of Glucose, Sodium, Potassium and Calcium Ions
Neutral sugar, monovalent and divalent cations were separated in HILIC/cation-exchange mode on a Primesep N mixed-mode column. Mobile phase is compatible with LC/MS and method can be used for analysis polar molecules in HILIC, cation-exchange or anion-exclusion modes. Application Analytes: Calcium Potassium Sodium Application Detection: ELSD/MS Detection |
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