Steroid

322

Synonyms

    Applications:

    Separation of Steroids in Reverse and Normal Mode on Primesep 100 Column

    October 15, 2005

    The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both reversed- and normal-phase modes. Switching modes by changing the mobile phase reverses peaks 2 and 3. The separation method uses a mobile phase mixture of water and acetonitrile (MeCN, ACN) for reversed phase and hexane and ethanol for normal phase with UV detection at 240 nm.

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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    Primesep C

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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    Application Analytes:
    Steroid

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.