Compounds  →  Sucrose

Separation of Sucrose

HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar



Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers.

Application Analytes:

Amino Acids
Polysorbate
Polysorbate 20
Polysorbate 80
Tween 20
Tween 80
Histidine
Glycine
Sucrose

Application Detection:

ELSD/MS Detection

HPLC Separation of Barium Cation in the Presence of Citric Acid, Sucrose and Tween 80

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Barium ion and citric acid are analyzed in the presence of sucrose and Tween 80 on Obelisc N HILIC mixed-mode column. Both compounds are retained in ion-exchange mode and HILIC is not employed with this method. Obelisc N HPLC column has basic and acidic ion-exchange groups separated by long hydrophilic chain. Presence of that long chain allows both cation- and anion-exchange mechanisms to exist simultaneously. Obelisc N column can be used for analysis of cation and anions in HILIC/ion-exchange mode and in ion-exchange mode. Analytes can be monitored by ELSD/CAD and LC/MS.



Application Analytes:

Citric Acid
Sucrose
Tween 80

Application Detection:


HPLC Separation of Sugars on Primesep S2 Column

Five sugars were separated on a Primesep S2 HILIC column with LC/MS compatible conditions. Various mobile phase produce different selectivity and separation. Method can be used as alternative to aminopropyl column. Primesep S2 column is stable and does not undergo rapid hydrolysis like aminopropyl columns.



Application Analytes:

Fructose
Glucose
Lactose
Maltose
Raffinose
Sorbitol
Sucrose
beta-D-Lactose
alpha-D-Lactose

Application Detection:

ELSD/MS Detection

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