Compounds  →  Sulfate

Separation of Sulfate

HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC



Sulfate and phosphate are separated on Primesep B2 and Primesep D column by anion-exchange mechanism. Because both sulfate and phosphate ions are not UV-active, ELSD is used to monitor separation of both anions. Method can be used to quantitate phosphate and sulfate in various pharmaceutical and chemical formulation, products and solution, drinking and ground water. Retention time is adjusted by increase or decrease of buffer concentrations. Two anions can be retained and separated on a very short column due to strong ion-exchange interaction. Method shows good reproducibility and versatility.
Application Analytes:

Sodium
Sulfate
Phosphate

Application Detection:

ELSD/MS Detection
HPLC Analysis of Sulfate and Phosphate Ions on Primesep Columns


Inorganic and organic ions are usually analyzed by ion-exchange mechanism. In this application, we used short Primesep B2 and Primesep D mixed-mode HPLC columns to retain and separate phosphate and sulfate ions. Both ions are retained by ion-exchange mechanism. Detection technique for analysis of these anions is Evaporative Light-Scattering Detector (ELSD).


Application Analytes:

Phosphate
Sulfate

Application Detection:

ELSD/MS Detection
HPLC Separation of Sulfamic and Sulfuric Acid on Primesep N HILIC Column



Sulfuric acid (sulfate ions) and sulfamic acid are analyzed in HILIC mode on Primesep N column. Both compounds are hydrophilic in nature and lack UV activity. Compounds are retained by hydrophilic interaction mechanism. Sulfate ion and sulfamic acid are monitored by ELSD. Method can be used for analysis of other inorganic ions.
Application Analytes:

Sulfamic Acid
Amidosulfonic Acid
Aminosulfonic Acid
Sulfate

Application Detection:

ELSD/MS Detection
Simultaneous Determination of Sodium and Sulfate Ions on Obelisc N Mixed-mode HILIC Column



Inorganic cations and anions are usually analyzed by two different methods - cation-exchange and anion-exchange. This requires development and validation of two methods. Both ions can be analyzed on mixed-mode HILIC column with ELSD detection. Both ions are retained by combination of HILIC and ion-exchange mechanism.
Application Analytes:

Sodium
Sulfate

Application Detection:

ELSD/MS Detection
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