Tetraethylammonium

CAS Number66-40-0
Molecular FormulaC8H20N
Molecular Weight130.254
InChI KeyCBXCPBUEXACCNR-UHFFFAOYSA-N
LogP-1.99
Synonyms
  • Tetraethylammonium
  • N,N,N-Triethylethanaminium
  • Ethanaminium, N,N,N-triethyl-
  • 66-40-0
  • 4-04-00-00331
  • BRN 1738225
  • Tetramon
  • Tetrylammonium
  • Tetraetilammonio
  • UNII-5AV7G7EIEE

Applications:

HPLC Method for Analysis of Tetraethylammonium Chloride on Newcrom AH Column

July 11, 2023

HPLC Method for Analysis of Tetraethylammonium on Newcrom AH by SIELC Technologies

HPLC Method for Analysis of Tetraethylammonium on Newcrom AH by SIELC Technologies

Tetraethylammonium is a quaternary ammonium cation with the chemical formula (C2H5)4N+. This compound consists of a central nitrogen atom surrounded by four ethyl groups.

As with other quaternary ammonium compounds, the nitrogen atom in tetraethylammonium carries a positive charge. This compound is often used in its salt forms, such as tetraethylammonium bromide or tetraethylammonium chloride.

Tetraethylammonium has a variety of uses in both the scientific and industrial domains. In biological research, it’s commonly used to block potassium channels in cell membranes, which allows researchers to study these channels in isolation. It’s also utilized in organic chemistry as a phase transfer catalyst, facilitating reactions between compounds in different phases.

The tetraethylammonium can be retained, analyzed, on a Newcrom AH column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium formate (AmFm) ionic modifier. This analysis method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Tetraethylammonium

Condition

ColumnNewcrom AH, 4.6 x 150 mm, 3 µm, 100 A
Mobile PhaseMeCN/H2O – 50/50%
BufferAmFm pH 3.0- 10 mM
Flow Rate1.0 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 50 °C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
(MS- compatible mobile phase)
Peak Retention Time7.12 min
Sample concentration1 mg/ml
Injection volume1 µl
LOD 320 ppb

Description

Class of CompoundsHydrophilic, Metal, Ion, Quaternary ammonium salt
Analyzing CompoundsTetraethylammonium

Application Column

Newcrom AH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A

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Application Analytes:
Tetraethylammonium

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Quaternary Amines

September 11, 2003

Primesep C separates a mixture of quaternary amines by a combination of cation exchange, complex formation, and hydrophic interactions. Methyltriethylammonium, tetraethylammonium, and tetramethylammonium cations as well as bromide counter ion are separated on a short 50 mm column. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and triethylamine acetate with evaporative light scattering detection (ELSD).

Condition

Column Primesep C, 4.6×50 mm
Mobile Phase MeCN/H2O
Buffer TEA acetate
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Quaternary Amines, Ionizable, Hormone
Analyzing Compounds Methyltriethylammonium, tetraethylammonium, Tetramethylammonium

 

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Bromide
Methyltriethylammonium Bromide
Quaternary Amines
Tetraethylammonium
Tetramethylammonium

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.