Tricine

62

CAS Number5704-04-1
Molecular FormulaC6H13NO5
Molecular Weight179.172
InChI KeySEQKRHFRPICQDD-UHFFFAOYSA-N
LogP-1.67
Synonyms
  • Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-
  • N-[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]glycine
  • 5704-04-1
  • EINECS 227-193-6
  • NSC 369995
  • UNII-W12LH4V8V3
  • N-(tri(hydroxymethyl)methyl)glycine
  • N-tris(hydroxymethyl)methylglycine
  • Tricine

Applications:

Uv-Vis Spectrum of Tricine

January 9, 2026
Access the UV-Vis Spectrum SIELC Library
UV-Vis Spectrum of Tricine.

If you are looking for optimized HPLC method to analyze Tricine check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

 

Application Analytes:
Tricine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

TFA Concentration Effect on Retention of Tricine

April 5, 2004

Primesep A retains the hydrophilic zwitterion tricine by a combination of polar interactions and ion exchange. Tricine is not retained by traditional reversed-phase chromatography. The retention on Primesep A is adjustable by changing the concentration of TFA in the mobile phase. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).

Application Column

Primesep A

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Tricine
Zwitterion
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.