Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Triton X100 check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.

HPLC Method for Separation of Hydrophobic, Cationic, Nonionic and Anionic Surfactants on Newcrom BH by SIELC Technologies

Surfactants, also known as surface-active agents, are compounds that lower the surface tension (or interfacial tension) between two liquids or between a liquid and a solid. Surfactants may act as detergents, wetting agents, emulsifiers, foaming agents, or dispersants.

They are often classified according to the charge of the polar head group:

Anionic Surfactants: These surfactants have a negative charge on their polar head group. Common examples include soap, sodium laureth sulfate, and sodium lauryl sulfate. They are commonly used in detergents and shampoos due to their ability to emulsify oils and hold dirt in suspension, so it can be rinsed away.

Cationic Surfactants: These surfactants have a positive charge on their polar head group. Examples include cetyltrimethylammonium bromide (CTAB) and benzalkonium chloride. These are often used as antiseptics and can also be found in hair conditioners because they reduce static cling.

Nonionic Surfactants: These surfactants have no charge on their polar head group. Examples include alcohol ethoxylates, nonylphenol ethoxylates, and polysorbates. Nonionic surfactants are often used in laundry and dishwasher detergents.

Sodium, which  has a chemical symbol of Na, is a soft alkali metal that is highly reactive.  It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial  for the body’s function and fluid balance.

Benzalkonium chloride, also known as Zephiran or Alkyldimethylbenzylammonium chloride, is a positively charged surfactant with the chemical formula C₆H₅CH₂N(CH₃)₂RCl. It is often used as a pharmaceutical preservative and as an antiseptic. You can find detailed UV spectra of Benzalkonium chloride and information about its various lambda maxima by visiting the following link.

Cetylpyridinium Chloride, also written as CPC, is a cationic quaternary ammonium compound with the chemical formula C₂₁H₃₈ClN. It is commonly used in mouthwashes, toothpastes, lozenges, throat sprays, breath sprays, and nasal sprays due to it’s antiseptic abilities. You can find detailed UV spectra of Cetylpyridinium Chloride and information about its various lambda maxima by visiting the following link.

Triton X100 is a nonionic surfactant with the chemical formula C₁₄H₂₂O(C₂H₄O)_n. It is primarily used as a detergent in laboratories, but it has a wide variety of laboratory uses. Commercially, it can be found in cleaning compounds. You can find detailed UV spectra of Triton X100 and information about its various lambda maxima by visiting the following link.

Chloride is typically used to refer to a compound or molecule that contains a chlorine anion: Cl^–. It is an electrolyte that is essential for bodily functions including blood pH, fluid balance, cellular functions, and more. Imbalance of chloride can indicate underlying health problems.

1-Pentanesulfonic acid is an organosulfonic acid with the chemical formula C₅H₁₂O₃S. It is primarily used in it’s sodium salt form as an anionic pairing agent. You can find detailed UV spectra of 1-Pentanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Hexanesulfonic acid is an alkyl sulfonate compound with the chemical formula C₆H₁₃O₃S^– (or C₆H₁₃NaO₃S as a sodium salt). It is primarily used in it’s sodium salt form as an ionic pairing agent in high-performance liquid chromatography.

1-Heptanesulfonic acid is an organosulfonic acid with the chemical formula C₇H₁₆O₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Heptanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Octanesulfonic acid is an anionic organosulfonic acid with the chemical formula C₈H₁₈O₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Heptanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Decanesulfonic acid is an alkyl sulfonic acid with the chemical formula C₁₀H₂₁NaO₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Decanesulfonic acid and information about its various lambda maxima by visiting the following link.

Sodium dodecyl sulfate (SDS), or sodium lauryl sulfate (SLS), is a surfactant used in cleaning, hygienic, and food products. It has a chemical formula C₁₂H₂₅NaSO₄, meaning it has twelve carbon hydrophobic tail attached to a polar sulfate. At low concentrations, it is used as a whipping aid and emulsifier in foods containing egg whites. It has a similar use in pharmaceutics as an ionic solubilizer and, also, an emulsifier. In laboratory uses, it is used as a component for lysing cells during RNA extraction or DNA extraction. You can find detailed UV spectra of Sodium dodecyl sulfate and information about its various lambda maxima by visiting the following link.

Benzalkonium chloride, Cetylpyridinium Chloride, Triton X100, 1-Pentanesulfonic acid, 1-Hexanesulfonic acid, sodium salt, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, Sodium dodecyl sulfate, 1-Octanesulfonic acid can be retained, separated, and analyzed using a reverse-phase Newcrom BH, 4.6 x 250 mm, 5 µm, 100 A, dual ended column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Ammonium formate, which serves as a buffer. This analytical method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

Condition

Description

Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. SHARC 1 HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved in anhydrous conditions by hydrogen bonding using acetonitrile (ACN) and methanol (MeOH) as the mobile phase with formic acid and ammonium formate (AmFm) as buffer. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (UV, ELSD, LC/MS, RI, CAD, etc.)

Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. Obelisc N HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved by hydrophilic interaction chromatography. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (ultra-violet, ELSD, LC/MS, RI, CAD, etc.)

Surfactants are molecules that contain both hydrophilic and hydrophobic groups, usually in the form of a hydrophilic head and a hydrophobic tail. Surfactants are used in detergents where they can form micelles around hydrophobic dirt molecules and wash them away. Triton X-100 is a surfactant with a hydrophilic polyethylene oxide chain that can be separated on a Primesep D reverse-phase HPLC column based on the number of oxide units in the chain. The mobile phase is water, acetonitrile (MeCN, ACN) and sulfuric acid as buffer. UV detection at 210nm.