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Separation of Tyrosine Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds  Primesep 100 separates a mixture of amino acids (tyrosine, phenylalanine), organic acids (benzoic acid, mandelic acid), amines (benzylamine, pyridine), and neutrals (benzonitrile, toluene) in one HPLC run by combining reversed-phase, cation-exchange, and polar interactions. The method is tunable and peak order can be changed significantly by adjusting acetonitrile and trifluoroacetic acid concentrations. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and compatible with UV, mass spec (LC/MS) and evaporative light scattering (ELSD) detection. Application Analytes: Amino Acids Benzoic Acid Benzonitrile Benzylamine Mandelic Acid Phenylalanine Pyridine Toluene Tyrosine Application Detection: UV Detection Catecholamine Pathway  Primesep 200 separates catecholamines in the catecholamine pathway in 10 minutes. Phenylalanine, tyrosine, DOPA, dopamine, norepinephrine, and epinephrine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Phenylalanine Tyrosine DOPA (3,4-dihydroxy-L-phenylalanine) Dopamine Epinephrine Application Detection: UV Detection Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (1)  Application Analytes: Phenylalanine Tyrosine DOPA (3,4-dihydroxy-L-phenylalanine) Application Detection: UV Detection HPLC Analysis of Polar Basic and Acetic Compounds on Primesep AB Column
Application Analytes: Benzoic Acid Dopamine Maleic Acid Tyrosine Application Detection: UV Detection HPLC Separation of Compounds of Catecholamine Pathway  The catecholamine neurotransmitters are amino acid derivatives of tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine and baseline resolved on a Primesep 200 column. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), trifluoracetic acid (TFA) or ammonium formate and ultraviolet (UV) detection at 210 nm. Peak order and retention time can be changed by switching from TFA to ammonium formate in the mobile phase. Application Analytes: DOPA (3,4-dihydroxy-L-phenylalanine) Tyrosine Phenylalanine Epinephrine Dopamine Application Detection: UV Detection ELSD/MS Detection Separation of Catecholamines on Primesep 100 Column with Phosphate Buffers
The catecholamine neurotransmitters are amino-acid derivatives of tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine and baseline are resolved on a Primesep 100 column with UV-transparent phosphate buffer. This method can be used for analysis of catecholamines and related impurities in various matrices. Peak order and retention time can be changed by changing the amount of ACN, buffer concentration and buffer pH. Various buffers can be used to accommodate desired detection technique. Primesep 100 is a reversed-phase cation-exchange mixed-mode column that can be used for analysis of polar neutral, polar ionizable, polar zwitter-ionic, hydrophobic neutral, and hydrophobic ionic compounds in the same run. Column can be operated in reverse-phase, cation-exchange, anion-exclusion, HILIC and mixed-modes depending on the mobile phase selection and nature of analytes. Column is compatible with LC/MS and does not require use of ion-pairing reagents. Application Analytes: DOPA (3,4-dihydroxy-L-phenylalanine) Dopamine Epinephrine Norepinephrine Phenylalanine Tyrosine Application Detection: UV Detection Separation of Amino Acids in Zero Organic Mode on Primesep 200 column
Essential and non-essential amino acids can be retained and separated in zero-organic mode on Primesep mixed-mode HPLC columns. Zero-organic mode is required to monitor isotopes of carbon. Amino acids are retained by combination of reversed-phase and cation-exchange mechanisms. At lower pH, some of the amino acids are more hydrophobic. Buffer pH will affect ionization state of amino acids, and at higher pH (above 2.5), the amino acids will be less hydrophobic and retentive in zero-organic mode. Amino acids can be monitored by low UV. Method can be used in archeological research for analysis of various molecules where presence of organic component of the mobile phase interferes with analysis. Application Analytes: Histidine Isoleucine Leucine Lysine Phenylalanine Tyrosine Valine Application Detection: UV Detection Separation of Amino Acids in HILIC and Cation-Exchange Modes
Amino acids can be retained and separated on a Primesep S2 column in HILIC/cation-exchange mode. Application Analytes: DOPA (3,4-dihydroxy-L-phenylalanine) Phenylalanine Tyrosine Application Detection: UV Detection |
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