Compounds  →  Uridine

Separation of Uridine

HPLC Separation of Uridine and Uracil


Uracil is a naturally occurring pyrimidine derivative. Uracil is used for drug delivery and as a pharmaceutical. Uridine is a product of uracil and sugar ribose. Uridine is one of components of ribonucleic acid. Uracil and uridine are slightly basic in nature. Both compounds are hydrophilic and uracil is very often is used as a void marker in reversed-phase chromatography. Uracil and uridine are separated based on their polar properties on a Primesep N HILIC column. Uracil and uridine are monitored by UV.


Application Analytes:

Uracil
Uridine

Application Detection:

UV Detection
HPLC Separation of Nucleic Bases at pH 4 and 5 on Obelisc N




Application Analytes:

Cytosine
Uracil
Uridine
Cytidine
Adenosine
Guanosine

Application Detection:

UV Detection
HILIC Retention of Uridine and Uracil on Sielc's HILIC Columns



Uridine and uracil are separated on three HILIC columns from SIELC. Primesep N, Primesep S, and Obelisc N columns all have different polarity and different embedded acidic groups on the surface of silica gel: Primesep N is a normal-phase HPLC column with embedded acidic groups with a pKa of about 5. Primesep S is a normal-phase HPLC column with embedded acidic groups with a pKa of about 3. The Primesep S stationary phase retains basic compounds by cation-exchange at pH > 3.

In traditional HILIC mode, a charged or neutral polar analyte interacts with a water layer on the polar stationary phase surface. On Obelisc N the charges are greatly separated and independently accessible, resulting in different selectivity compared to traditional HILIC and silica columns. Mobile phase composition changes the conformation of the long hydrophilic chain. All three columns can be used to analyze uridine and uracil by HPLC with UV, LC/MS and ELSD detection.
Application Analytes:

Uridine
Uracil

Application Detection:

UV Detection
Separation of Uracil and Uridine in HILIC Mode on Primesep S2 Column

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Uracil and uridine are separated based on their polarity. The mechanism of retention is pure HILIC mode. Retention time is controlled by varying the amount of ACN. Buffer concentration and pH will affect ionization and polarity of the stationary phase, and thus indirectly affect retention of neutral compounds. Column is compatible with all general detection techniques.


Application Analytes:

Uracil
Uridine

Application Detection:

UV Detection
ELSD/MS Detection
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