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Separation of Vitamin B1 (Thiamine) Separation of Vitamin B  Primesep C separates B vitamins with baseline resolution by a combination of cation exchange, complex formation, and hydrophobic interactions. Vitamin B1 (thiamine), vitamin B2 (riboflavin), and vitamin B6 (pyridoxine) are separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 280 nm. Application Analytes: Vitamin B1 (Thiamine) Vitamin B2 (Riboflavin) Vitamin B3 (Niacin) Riboflavin (Vitamin B2) Thiamine (Vitamin B1) Pyridoxine Vitamins Application Detection: UV Detection HILIC Separation of Vitamins Group B  Separation of vitamins group B is achieved on Obelisc N column in HILIC mixed-mode. Vitamins of this group are different in polarity and ionic properties. Retention and separation is achieved by optimization of amount of ACN, buffer and buffer pH. Combination of UV and ELSD detection is used to monitor HPLC separation. B vitamins are water-soluble vitamins that play an important role in cell metabolism. Supplements containing all six are generally referred to as a vitamin B complex. Individual B vitamin supplements are referred to by the specific name of each vitamin. This method can be used to analyze individual B vitamins as well as vitamin B complex. Isolation of impurities as well as degradation products is possible by preparative chromatography. Application Analytes: Vitamin B3 (Niacin) Vitamin B6 (Pyridoxine) Vitamin B2 (Riboflavin) Vitamin B5 (Pantothenic Acid) Vitamin B1 (Thiamine) Nicotinic Acid Application Detection: UV Detection ELSD/MS Detection |
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