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Separation of Vitamin B6 (Pyridoxine) HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities  Vitamin C (ascorbic acid) and Vitamins Group B are separated on Obelisc N mixed-mode column. Method can be used in quantitation and determination of polar vitamins in various formulations and dietary supplements. HPLC method can be based on UV, Evaporative Light Scattering Detection (ESLD), RI or MS detection. Effect of sample matrix can be eliminated by changing mobile phase conditions. Buffer concentration, buffer pH and amount of ACN will affect every vitamin differently due to difference in polar and ionic properties. Application Analytes: Pyridoxine Vitamin B6 (Pyridoxine) 4-Aminopyridine/Fampridine Vitamin B2 (Riboflavin) 3-Aminopyridine Isonicotinic Acid Nicotinic Acid Barbituric Acid Ascorbic Acid Application Detection: UV Detection HILIC Separation of Vitamins Group B  Separation of vitamins group B is achieved on Obelisc N column in HILIC mixed-mode. Vitamins of this group are different in polarity and ionic properties. Retention and separation is achieved by optimization of amount of ACN, buffer and buffer pH. Combination of UV and ELSD detection is used to monitor HPLC separation. B vitamins are water-soluble vitamins that play an important role in cell metabolism. Supplements containing all six are generally referred to as a vitamin B complex. Individual B vitamin supplements are referred to by the specific name of each vitamin. This method can be used to analyze individual B vitamins as well as vitamin B complex. Isolation of impurities as well as degradation products is possible by preparative chromatography. Application Analytes: Vitamin B3 (Niacin) Vitamin B6 (Pyridoxine) Vitamin B2 (Riboflavin) Vitamin B5 (Pantothenic Acid) Vitamin B1 (Thiamine) Nicotinic Acid Application Detection: UV Detection ELSD/MS Detection |
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