Compounds  →  Vitamin E Acetate

Separation of Vitamin E Acetate

Separation of Vitamin E and Vitamin E Acetate



Primesep B separates Vitamin E and Vitamin E acetate with reversed peak order of a traditional C8 column. The different selectivity is due to an embedded basic functional group on the stationary phase which contributes polar interactions to the separation. Excellent peak shape results with a mass spec compatible mobile phase of water and acetonitrile (MeCN, ACN) with UV detection at 205 nm.
Application Analytes:

Vitamin E
Vitamin E Acetate

Application Detection:

UV Detection
HPLC Analysis of ACE Serum Active Ingredients


Mixed-mode HPLC columns allow for separation of compounds with drastically different properties. In this application, highly hydrophilic ascorbic acid is retained and separated from highly hydrophobic retinol and Vitamin E Acetate. Ascorbic acid is retained by anion-exchange mechanism and hydrophobic compounds are retained by reversed-phase mechanism. Compounds are monitored by UV detection. Mobile phase is compatible with LC/MS and method can be used to monitor basic and acidic compounds in bio-fluids (serum, blood, urine, saliva, etc.)


Application Analytes:

Ascorbic Acid
Vitamin E Acetate

Application Detection:

UV Detection
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