| CAS Number | 87-99-0 |
|---|---|
| Molecular Formula | C5H12O5 |
| Molecular Weight | 152.146 |
| InChI Key | HEBKCHPVOIAQTA-SCDXWVJYSA-N |
| LogP | -2.77 |
| Synonyms |
|
Applications:
HPLC Method For Simultaneous Quantification of Sugar Alcohols (Polyols) on Primesep AP
September 9, 2021
Separation type: Liquid Chromatography HILIC
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylene glycol, Glycerol, Erythritol, Xylitol, Sorbitol.
| Column | Primesep AP, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 75/25% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm, RI |
| Class of Compounds |
Drug, Basic, Hydrophobic, Ionizable, Zwitterionic |
| Analyzing Compounds | Ethylene glycol, Glycerol, Erythritol , Xylitol, Sorbitol |
Application Column
Primesep AP
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsEthylene Glycol
Glycerol
Sorbitol
Xylitol
HPLC Analysis of Xylitol on Primesep S Column
July 3, 2013
Xylitol is a polyalcohol which is used as a sweetener for people with diabetes. Xylitol is naturally found in the fibers of fruits and vegetables. It is a very highly polar, non-ionizable molecule. Xylitol can be well retained on the Primesep S HILIC column. It has a relatively high water concentration in the mobile phase that helps to dissolve xylitol at high acetonitrile HILIC applications. Polar surface on the Primesep S column provides well controlled retention time and can be used as a robust method for analysis of various sugar-lie molecules.
| Column | Primesep S, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | AmFm pH 3.0- 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Sugar, Hydrophilic, Ionizable |
| Analyzing Compounds | Xylitol |
Application Column
Primesep S
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
HPLC Analysis on Xylose and Xylitol on Primesep S Column
July 3, 2013
Xylose is a monosaccharide which can be found in wood. Xylitol is a sugar-like, open chain polyalcohol. Xylitol and xylose were separated on the Primesep S HILIC/mixed-mode column. Both compounds are retained by a single HILIC mechanism. This method is compatible with ELSD, CAD, and RI detection and can be used for analysis of various monosaccharides in complex mixtures.
| Column | Primesep S, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | AmFm pH 3.0- 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Sugar, Hydrophilic, Ionizable |
| Analyzing Compounds | Xylose, Xylitol |
Application Column
Primesep S
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsXylose
