HPLC Analysis of Piperazine on Primesep Columns

 

Application Notes: Piperazine is an organic hydrophilic basic compound containing two secondary amines. It is not retained on traditional reversed-phase columns without ion pairing reagent. Since piperazine is not UV active, alternative detection techniques like ELSD, CAD and LC/MS must be employed. Two fast methods of analysis on Primesep 100 and 200 columns were developed using reversed-phase cation-exchange approach. Primesep 200 column is more suitable for analysis since piperazine interacts strongly with stationary phase and since Primesep 200 columns are weaker columns. Methods can be used for analysis of piperazine and other basic hydrophilic compounds in reaction mixtures, formulations, biofluids, waste waters, etc. This Method is compatible with ELSD, CAD and LC/MS.

Application Columns: Primesep 100, Primesep 200
Application compounds: Piperazine
Detection technique: UV, LC/MS, ELSD/CAD

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Piperazine

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.