HPLC Method for Analysis of Hyoscyamine

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Hyoscyamine is a natural plant belladonna alkaloid derivative and the levorotatory form of racemic atropine. It functions as a non-selective, competitive antagonist of muscarinic receptors and is used to treat a variety of stomach pains and intestinal problems as well as to decrease side effects of certain medications and insecticides. The drug does that by decreasing the acid production in the stomach and slowing down the natural movements of the gut. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Hyoscyamine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

 

Condition

Column Primesep 200, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN – 40%
Buffer H3PO4 – 0.1%,
Flow Rate 0.5 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug,  Acid, Hydrophilic, Ionizable, Supplements
Analyzing Compounds Hyoscyamine

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Hyoscyamine
Hyoscyamine Sulfate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.