Prednisolone is a synthetic glucocorticoid with anti-inflammatory and immunomodulating properties. It can bind to and activate specific nuclear receptors, resulting in an altered gene expression and inhibition of proinflammatory cytokine production. Prednisolone is used to treat different conditions (blood problems, arthritis, immune system disorders). Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Ofloxacin. The method is UV compatible and can be used as a general approach for analyzing similar compounds.