HPLC Separation of Fungicides in a Green Tea Matrix

 

Even though teas are known for containing polyphenols such as catechins, flavonoids and theaflavins, SIELC mixed-mode columns can be used to analyze a matrix from a commercial tea and retain target compounds. In this method, green tea was spiked with 0.005mg/ml of pesticides previously found in teas. Primesep 200 was used for it’s reverse-phase and acidic ion-pairing groups. Target compounds were retained and resolved from the majority of the tea matrix peak.

 

Condition

Column Primesep 200, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H3PO4 – 0.05 %
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Statin, Hydrophobic, Ionizable
Analyzing Compounds Atorvastatin, Picoxystrobin, Pyrimethanil, Lipitor

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Atorvastatin
Picoxystrobin
Pyrimethanil

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.